中华眼镜蛇毒F组分抗血小板聚集的作用机制

目的探讨中华眼镜蛇毒F组分抑制血小板聚集的作用机制。方法用比浊法测定中华眼镜蛇毒F组分对二磷酸腺苷、花生四烯酸和血小板活化因子诱导血小板聚集作用的影响,流式细胞术观察中华眼镜蛇毒F组分对荧光标记的单克隆抗体CD41(FITC-CD41)和CD61(FITC-CD61)与血小板膜糖蛋白IIb/IIIa(GPIIb/IIIa)结合的影响。结果中华眼镜蛇毒F组分明显抑制二磷酸腺苷、花生四烯酸和血小板活化因子诱导的血小板聚集,其作用呈现一定程度的剂量依赖关系。中华眼镜蛇毒F组分可以明显降低单克隆抗体CD41(抗GPIIb)与血小板的结合率,而对单克隆抗体CD61(抗GPIIIa)与血小板的结合率没有影响。结论中华眼镜蛇毒F组分可以抑制多种激动剂诱导的血小板聚集,其机制和中华眼镜蛇毒F组分与血小板膜糖蛋白IIb/IIIa复合物的结合有关。

Inhibitory mechanism of fraction F of Naja naja atra venom on platelet aggregation

Objective To explore the inhibitory mechanism of fraction F of Naja naja atra venom on platelet aggregation.Methods Nephelometery was used to detect the effect of fraction F of Naja naja atra venom on platelet aggregation induced by adenosine diphosphate (ADP),arachidonic acid (AA) and platelet activating factor (PAF).The influence of fraction F of Naja naja atra venom on the binding of fluoreacence-conjugated monoclonal antibody CD41 (FITC-CD41) and CD61 (FITC-CD61) to membrane glycoprotein IIb/IIIa (GPIIb/IIIa) of platelet was measured by flow cytometry.Results Fraction F of Naja naja atra venom significantly inhibited the aggregation of platelet induced by ADP,AA and PAF in a dose-dependent manner.Fraction F of Naja naja atra venom obviously decreased the binding of FITC-CD41 to GPIIb,and had no effect on the binding of FITC-CD61 to GPIIIa.Conclusion Fraction F of Naja naja atra venom can inhibit the platelet aggregation induced by different agonists,which is one of mechanisms underlying fraction F of Naja naja atra venom binding to GPIIb/IIIa of platelet.