P38信号通路在人脐静脉内皮细胞表达P-选择素中的作用

目的 :研究P38信号通路 (P38mitogen activatedproteinki nase ,P38MAPK)在人脐静脉内皮细胞表达P 选择素以及糖尿病动脉粥样硬化中的作用。方法 :分别以高葡萄糖、糖基化终产物 (AGE)、高胰岛素和过氧化氢刺激人脐静脉内皮细胞系ECV 30 4 ,再以P38MAPK特异性抑制剂SB2 0 35 80预处理 ,观察ECV 30 4细胞系中磷酸化P38MAPK和P 选择素的表达。结果 :4种刺激因素均可单独激活P38MAPK ,使磷酸化P38MAPK表达量明显增加 ,P 选择素表达量也明显增加 ;以SB2 0 35 80预处理后 ,P 选择素的表达被明显抑制。结论 :P38MAPK是P 选择素的上游信号分子 ,也可能是动脉粥样硬化发生的始动信号之一

Role of P38 mitogen-activated protein kinase in expression of P-selectin on human umbilical vein endothelial cells

AIM: To investigate the role of P38 mitogen activated protein kinase (P38MAPK) in expression of P selectin on human umbilical vein endothelial cells and diabetic atherosclerosis. METHODS: Human umbilical vein endothelial cell line ECV304 were stimulated with high glucose, advanced glycosylation end products (AGE), high insulin and H 2O 2 respectively , and then expression of phosphorylated P38MAPK and P selectin was detected after being pre treated with SB203580 (P38MAPK specific inhibitor). RESULTS: High glucose, AGE, high insulin and H 2O 2 could activate P38MAPK by oneself, and increased notably expression of phosphorylated P38MAPK and P selectin on cell line ECV304; Expression of P selectin was inhibited significantly by SB203580. CONCLUSION: P38MAPK can regulate the expression of P selectin, P38MAPK is an upstream signaling molecule and also may be one of initial signals on atherosclerosis occurrence.