| 阿魏酸对人脐静脉血管内皮细胞ECV304的增殖作用 |
| |
| 引用本文： | 王筠,袁卓,张军平. 阿魏酸对人脐静脉血管内皮细胞ECV304的增殖作用[J]. 中医药学报, 2007, 35(3): 4-6 |
| |
| 作者姓名： | 王筠 袁卓 张军平 |
| |
| 作者单位： | 1. 天津中医药大学,天津,300193;北京中医药大学,北京,100029
2. 天津中医药大学,天津,300193 |
| |
| 基金项目： | 国家自然科学基金;高等学校博士学科点专项科研项目 |
| |
| 摘 要： | 目的：观察阿魏酸对于培养人脐静脉内皮细胞ECV304增殖的影响,为治疗性血管新生的靶基因选择提供依据。方法：取对数生长期的内皮细胞,分为阿魏酸低、中、高浓度组,同步化于G0期,ELISA法检测BrdU的掺入;RT-PCR法检测血管内皮细胞生长因子（VEGF）mRNA的表达情况。结果：与对照组相比,不同浓度阿魏酸均可显著提高BrdU的掺入量,上调VEGFmRNA的表达,且在10^2ng/ml～10^4ng/ml之间呈一定的量效关系。结论：阿魏酸能显著促进体外培养的ECV304细胞的增殖,可能具有血管新生的作用。
|
| 关 键 词： | 阿魏酸 内皮 细胞 增殖 血管内皮细胞生长因子 |
| 文章编号： | 1002-2392（2007）03-0004-03 |
| 修稿时间： | 2006-12-30 |
Proliferation effect of Ferulic acid on endothelial cells ECV304 of human umbilical vein |
| |
| WANG Jun,YUAN Zhuo,Zhang Jun-ping. Proliferation effect of Ferulic acid on endothelial cells ECV304 of human umbilical vein[J]. Acta Chinese Medicine and Pharmacology, 2007, 35(3): 4-6 |
| |
| Authors: | WANG Jun YUAN Zhuo Zhang Jun-ping |
| |
| Abstract: | Objective:To observe the proliferation effect of ferulic acid on the endothelial cells ECV304 of human umbilical vein, so as to find a candidate way for therapeutic angiogenesis. Method:We incubated cells with FA (102ng/ml, 103ng/ml, 104ng/ml) for 24 h after synchronizing the ECV304 at G0, BrdU-ELISA assay was performed to test the DNA synthesis. The expression of VEGF mRNA by RT-PCR was also observed. Result:Compared with control, different concentration of ferulic acid can improve the BrdU incorporation and enhance the expression of VEGF mRNA with a dose-dependent manner raging from 102ng/ml to 104ng/ml. Conclusion:Ferulic acid can significantly improve the proliferation of ECV304 and play an important role in angiogenesis. |
| |
| Keywords: | Ferulic acid endothelial cells proliferation VEGF |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
