纳米羟基磷灰石-壳聚糖复合材料对细胞粘附的影响

目的：制备纳米羟基磷灰石-壳聚糖（nano hydroxyapatite-chitosan,nHA/CS）复合材料,观察其对成纤维细胞粘附行为的影响.方法：将溶胶-凝胶法制得的纳米羟基磷灰石充分混合于2%壳聚糖乙酸溶液中,冷冻干燥法制备纳米羟基磷灰石-壳聚糖复合材料,并对材料进行X射线衍射分析（XRD）、红外光谱分析（FT-IR）、扫描电镜（SEM）、透射电镜（TEM）检测.将NIH3T3细胞分别接种于复合材料和致密羟基磷灰石（dense hydroxyapatite,HA）表面,细胞计数法计算细胞粘附数量,扫描电镜下观察细胞粘附形态.结果：XRD分析结果表明溶胶-凝胶法制备的HA和nHA的晶体结构符合标准羟基磷灰石的空间6方晶体结构.TEM分析结果显示nHA/CS材料中羟基磷灰石为纳米级粉体.接种5、7、9 d后nHA/CS材料表面的细胞粘附数量高于HA组,差异有显著性（P＜0.05）.扫描电镜下,细胞以多个突起粘附于复合材料表面,并具有良好的伸展状态.结论：与HA相比,nHA/CS材料更有利于成纤维细胞的粘附,生物相容性良好.

The adhesive behavior of NIH3T3 cells to the nanometer hydroxyapatite/chitosan composite

Objective:To prepare nanometer hydroxyaptite/chitosan composite(nHA/CS)and evaluate its biocompa-tibility with fibroblasts.Method:The nanometer hydroxyapatite powder was prepared with the sol-gel method and then dispersed completely in 2% chitosan/acetic acid solution.The obtained composites were cryodesiccated and then measured for the characters with XRD,FT-IR,SEM,TEM.Attachment quantity and morphology of fibroblasts were investigated on nHA/CS and dense HA.Result:It was showed that in X-ray diffraction,the characteristically diffracted peaks of precipitated HA nanocrystals were identical with stoichimetric HA with reference to JCPDS.HA powder in composites was showed to be nanopaticles in transmission electron microscope.After co-cultured for 5 days,7 days and 9 days,the rate of attached cells on the scaffold materials was higher than that on dense HA.Conclusion:Compared with dense HA,the new scaffold material,nanohydroxyapatite/chitosan,has significant ability of adherence of fibroblasts and is believed to be considerable improving in the biocompatibility of hydroxyapatite.