Short communication: Molecular analysis of Potato leafroll virus isolates from the Czech Republic |
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Authors: | Helena Plchova Noemi Cerovska Tomas Moravec and Petr Dedic |
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Institution: | (1) Laboratory of Virology, Institute of Experimental Botany AS CR, v. v. i., Na Karlovce 1a, 160 00 Prague 6, Czech Republic;(2) Potato Research Institute, 580 01 Havlickuv Brod, Czech Republic |
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Abstract: | The complete genomes of three Czech isolates VIRUBRA 1/045, VIRUBRA 1/046, and VIRUBRA 1/047 of Potato leafroll virus (PLRV) were sequenced and compared with 13 complete sequences of PLRV isolates available in GenBank. Among the Czech isolates,
VIRUBRA 1/046 and 1/047 showed the highest nucleotide (nt) identity (98.7%). PLRV was the most conserved virus in both open
reading frames (ORFs) 3 and 4. The most variable regions were ORFs 0 and Rap1. Interestingly, isolate VIRUBRA 1/045 significantly
differed from the other two Czech isolates in ORFs 0 and 1. Moreover, we identified mutations in the amino acid (aa) sequences,
which were specific for the Czech isolates. Phylogenetic analysis based on ORF0 showed that the Czech isolates could be classified
in two of the three groupings of the phylogenetic tree obtained. This is the first report on sequence analysis of the genome
sequences of PLRV isolates from the Czech Republic.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | Potato leafroll virus Sequencing Phylogeny Czech Republic |
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