解偶联蛋白3基因启动子区-55（C〉T）多态与中国人静息能量消耗及体脂含量与分布的关系

目的 研究解偶联蛋白3基因（UCP3）启动子区-55（C〉T）多态与中国人静息能量消耗、体脂参数的关系。方法 在300名中国人（正常体重91人，超重／月巴胖209人）中，用聚合酶链反应一限制性片段长度多态性（polymemse chain reaction-restriction fragment length polymorphisms，PCR—RFLP）检测伙巧基因启动子区-55（C〉T）变异，并测定其静息能量消耗、体脂含量及分布。结果 UCP3基因启动子区-55（C〉T）多态基因型频率与肥胖及肥胖类型均无相关。正常体重组1T基因型者静息能量消耗水平显著高于CT及CC基因型者（P〈0．05）；超重／肥胖组各基因型者间比较亦有同样趋势。在超重／肥胖组，TT基因型者FM／FFM值与CT及CC基因型者差异有显著意义（P〈0．01）。结论 UCP3基因启动子区-55（C〉T）多态与中国人静息能量消耗相关，该变异可能通过对静息能量消耗的影响调节机体的能量代谢。

Effect of polymorphism of uncoupling protein 3 gene -55 (C>T) on the resting energy expenditure, total body fat and regional body fat in Chinese

OBJECTIVE: To investigate the relationship of the C to T variant at the -55 site of the promoter region of uncoupling protein 3 gene (UCP3) with the resting energy expenditure and the parameters of body fat in Chinese population. METHODS: Three hundred Chinese (91 normal weight subjects, 209 overweight/obesity subjects) were genotyped for the UCP3 gene -55(C>T) by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Resting energy expenditure (REE), fat mass (FM), fat free mass (FFM) and the parameters for regional adipose tissue distribution were measured. RESULTS: Genotype frequencies of UCP3 gene -55(C>T) were not associated with obesity and different types of obesity. The REE level in normal weight subjects with TT homozygotes was higher than that in those with CT heterozygotes and CC homozygotes (P=0.0200). Similar tendency was also observed in overweight/obesity subjects. The FM/FFM exhibited significant difference between the overweight/obesity subjects with a TT genotype and those with a CT or CC genotype (P=0.0096). CONCLUSION: The level of difference in REE caused by the polymorphism of promoter region of UCP3 -55(C>T) may play a role in energy metabolism in Chinese.