肿瘤坏死因子相关凋亡诱导配体对卵巢癌裸鼠移植瘤SKOV3细胞的促凋亡研究

目的 研究肿瘤坏死因子相关凋亡诱导配体(TRAIL)蛋白对SKOV3移植瘤细胞半胱天冬氨酸蛋白酶-3(Caspase-3)表达的影响及其与肿瘤细胞凋亡的关系.方法 建立雌性裸小鼠SKOV3移植瘤24只,随机分为4组,每组6只.TRAIL组单用重组人TRAIL蛋白(10μg/kg),顺铂(DDP)组单用DDP(3 mg/...

Research on the Impact of TRAIL on Apoptosis of SKOV3 Cell Line Ovarian Tumor Xenografts in Nude Mice

Objective To investigate the effects of Tumor necrosis factor-related apoptosis-inducing ligand（TRAIL） on the expression of Cysteine/aspartic acid specific protease-3（Caspase-3） in SKOV3 ovarian tumor cells and its relationship with the apoptosis of the ovarian tumor xenografts in nude mice.Methods Twenty-four nude mice with SKOV3 cell line ovarian tumor were randomly divided into four groups with 6 in each group.TRAIL（10 μg/kg） was given to the mice in the TRAIL group;DDP（3 μg/kg） was given to the mice in the DDP group;TRAIL（10 μg/kg） and DDP（3 μg/kg） were given to the mice in the TRAIL＋DDP group;and 0.5 mL of saline solution was give to the mice in the control group.The expression of Caspase-3 was detected with immunohistochemistry.The apoptosis index（AI） of cells was determined by Terminal deoxynucleotidyl transferase mediated-dUTP nick end labeling（TUNEL）.Results The expression of Caspase-3 in the TRAIL group（171.67±14.38）,DDP group（172.50±14.75）,and TRAIL＋DDP group（230.00±40.99） was significantly higher than that in the control group（135.83±16.25）（P0.05）.The apoptosis index for the control group,TRAIL group,DDP group and TRAIL＋DDP group was 16.67±5.43,33.17±8.42,24.33±4.59,and 40.50±6.16,respectively.The apoptosis index for the TRAIL group and the TRAIL＋DDP group was significantly higher than that in the control group and the DDP group（P0.05）.Conclusion Soluble TRAIL has an effect on enhancing the expression of Caspase-3 in implanted tumor in nude mice.TRAIL protein can inhibit the growth of SKOV3 cells in nude mice by inducing cell apoptosis.