| 靶向PKM2干扰质粒联合重组人血管内皮抑素治疗肺癌的实验研究 |
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| 引用本文： | Mao JZ,Guo WH,Shi HS,Zhao YW,Yang HS. 靶向PKM2干扰质粒联合重组人血管内皮抑素治疗肺癌的实验研究[J]. 四川大学学报(医学版), 2011, 42(3): 308-312 |
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| 作者姓名： | Mao JZ Guo WH Shi HS Zhao YW Yang HS |
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| 作者单位： | 四川大学华西医院,生物治疗国家重点实验室,成都,610041 |
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| 摘 要： | 目的 研究丙酮酸激酶同工酶M2(PKM2)干扰质粒联合重组人血管内皮抑素是否能增强单药的抗癌作用.方法 免疫荧光检测A549细胞内PKM2蛋白表达,蛋白印迹检测PKM2质粒干扰效果.建立裸鼠皮下A549肺癌模型,将25只荷瘤小鼠随机平均分为5组:生理盐水组(NS组)、无关序列干扰质粒组(psh-Control组)、PK...
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| 关 键 词： | 丙酮酸激酶同工酶M2 RNA干扰 肺癌 抗血管生成 |
Antitumor effect by combination of shRNA interfering plasmid targeting PKM2 with recombinant endostatin |
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| Mao Jing-Zhi,Guo Wen-Hao,Shi Hua-Shan,Zhao Yu-Wei,Yang Han-Shuo. Antitumor effect by combination of shRNA interfering plasmid targeting PKM2 with recombinant endostatin[J]. Journal of Sichuan University. Medical science edition, 2011, 42(3): 308-312 |
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| Authors: | Mao Jing-Zhi Guo Wen-Hao Shi Hua-Shan Zhao Yu-Wei Yang Han-Shuo |
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| Affiliation: | MAO Jing-zhi,GUO Wen-hao,SHI Hua-shan,ZHAO Yu-wei,YANG Han-shuo.State Key Laboratory of Biotherapy,West China Hospital,Sichuan University,Chengdu 610041,China |
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| Abstract: | Obiective To investigate the enhancement effect of the combination of shRNA interfering plasmid targeting PKM2 with recombinant Endostatin in the treatment of lung cancer.Methods Twenty five BABL/nu/nu mice bearing A549 lung cancer were divided into 5 groups(NS control,psh-Control,psh-PKM2 treated group,Endostar treated group,psh-PKM2+Endostar treated group) and treated with shRNA interfering plasmid targeting PKM2 and recombinant Endostatin respectively or in combination.The expression of PKM2 in A549 dete... |
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| Keywords: | PKM2 RNAi Lung cancer Anti-angiogenesis |
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