艾灸对克罗恩病大鼠结肠黏膜MCP-1和IL-8蛋白表达的影响

目的通过观察艾灸对克罗恩病（CD）大鼠结肠黏膜MCP—1和IL-8蛋白表达的影响，探讨艾灸对CD大鼠结肠黏膜免疫功能的作用及可能机制。方法采用国际公认的MorriS方法制备CD大鼠模型。将动物随机分为正常对照组、模型组、隔药灸组、温和灸组、烟条灸组和热水灸组，均选取天枢（双侧）治疗。采用免疫组化方法，观察大鼠结肠黏膜组织IL-8、MCP-1蛋白的表达。结果模型组大鼠结肠黏膜组织MCP-1和IL-8蛋白表达阳性目标总面积和积分光密度均明显高于正常对照组（P〈0．01），经隔药灸和温和灸治疗后，CD大鼠结肠黏膜组织MCP-1和IL-8蛋白表达阳性目标总面积和积分光密度均显著降低（P〈0．01）。结论隔药灸和温和灸能够下调CD大鼠结肠黏膜MCP-1和IL-8蛋白的表达。

Influence of Moxibustion on the Expressions of MCP-1 and IL-8 Proteins in the Colonic Mucosa of Crohn's Disease Rats

Objective To explore the action of moxibustion on the immunologic function of the colonic mucosa of crohn＇s disease （CD） rats and its possible mechanism by observing the influence of moxibustion on the expressions of MCP-1 and IL-8 proteins in the colonic mucosa of CD rats. Methods A rat CD model was made using international accepted Morris method. The animals were randomly allocated to normal control, model, medicinal moxibustion, mild moxibustion, moxa stick moxibustion and hot water moxibustion groups. The expressions of MCP-1 and IL-8 proteins in the colonic mucosa of the rats were examined by an immunohistochemical method. Results Both the total area and the integral optical density of the positive expressions of MCP-1 and IL-8 proteins in the colonic mucosa increased significantly in the model group of rats compared with the normal control group （P 〈0.01）. Both the total area and the integral optical density of the positive expressions of MCP-1 and IL-8 proteins in the colonic mucosa of CD rats decreased significantly after medicinal moxibustion and mild moxibustion （P〈0.01）. Conclusion Medicinal moxibustion and mild moxibustion can down-regulate the expressions of MCP-1 and IL-8 proteins in the colonic mucosa of CD rats.