子宫内膜异位症患者在位和异位内膜组织中TLR3的表达及意义

目的检测TLR3在正常子宫内膜、子宫内膜异位症(内异症)患者在位和异位内膜中的表达情况,探讨TLR3在内异症发病中的作用。方法选择内异症患者的卵巢异位内膜45例(异位内膜组)和相对应的在位子宫内膜32例(在位内膜组),对照组为32例非内异症患者子宫内膜组织。采用免疫组化方法 (EnVision二步法)检测各组内膜组织中TLR3的表达情况,以及TLR3在内异症不同临床分期的异位内膜组织中的表达差异。结果内异症患者异位内膜、在位内膜及正常对照组子宫内膜中,TLR3蛋白均有不同程度表达,主要表达于子宫内膜腺上皮和腔上皮。TLR3蛋白在内异症患者异位内膜、在位内膜的表达均低于正常对照组子宫内膜,其差异有统计学意义(P0.01),其中表达最低的是异位内膜,但与在位内膜的差异无统计学意义(P0.05)。TLR3蛋白在Ⅰ～Ⅱ期内异症患者异位内膜的表达与Ⅲ～Ⅳ期患者异位内膜的表达比较,其差异无统计学意义(P0.05)。结论在位及异位内膜组织中TLR3低表达可能参与了内异症发病,但与内异症的临床分期无关。

Expression and Significance of TLR3 in Eutopic and Ectopic Endometrium from Women with Endometriosis

Objective To investigate the expression of TLR3 in endometrium from women without endometfiosis and in eutopie and ectopie endometrium from women with endometrlosis, exploring the role of TLR3 in the occurrence and development of endometriosis. Methods Ectopic （ n = 45 ） and homologous eutopic endometrium （ n = 32） from women with endometriosis and endometrium from women without endometriosis （ n = 32） were used for immunohistochemical analysis of TLR3. Results All endometrial samples from patients with endometriosis and healthy controls showed cytoplasmic TLR3 immunoreactivifies mainly in the luminal and glandular epithelium. The expression of TLR3 in eutopic endometrium and the ectopic endometrium was weaker than the normal control group （ P〈0. 01 ）, but there was no significant difference between ectopic and eutopic endometrium of endometriosis （ P〉0. 05）. No statistically significant difference was found in the eetopic endometfial expression of TLR3 between Ⅰ-Ⅱ stage and Ⅲ-Ⅳ stage of endometriosis （ P 〉 0. 05 ）. Conclusion The decreased expression of TLR3 might play a role in the pathogenesis of endometriosis. There was no correlation between the expression of TLR3 and clinical stages.