Multimodality multidimensional image analysis of cortical and subcortical plasticity in the rat brain |
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Authors: | Dr. Alberto F. Goldszal Oleh J. Tretiak Demeral D. Liu Peter J. Hand |
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Affiliation: | (1) Imaging and Computer Vision Center, Drexel University, Philadelphia, PA;(2) Mahoney Institute of Neurological Science, University of Pennsylvania, Philadelphia, PA;(3) Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA;(4) National Institute on Aging, Gerontology Research Center, Laboratory of Personality and Cognition, National Institutes of Health, 4940 Eastern Avenue, Box 03, 21224 Baltimore, MD, U.S.A. |
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Abstract: | In this work, we developed and implemented a multimodality multidimensional imaging system which is capable of generating and displaying anatomical and functional images of selected structures and processes within a vertebrate's central nervous system (CNS). The functional images are generated from [14C]-2-deoxy-d-glucose (2DG) autoradiography whereas the anatomic images are derived from cytochrome oxidase (CO) histochemistry. This multi-modality imaging system has been used to study mechanisms underlying information processing in the rat brain. We have applied this technique to visualize and measure the plasticity (deformation) observed in the rat's whisker system due to neonatal lesioning of selected peripheral sensory organs. Application of this imaging system revealed detailed information about the shape, size, and directionality of selected cortical and subcortical structures. Previous 2-D imaging techniques were unable to deliver such holistic information. Another important issue addressed in this work is related to image registration problems. We developed an image registration technique which employs extrinsic fiduciary marks for alignment and is capable of registering images with subpixel accuracy. It uses the information from all available fiduciary marks to promote alignment of the sections and to avoid propagation of errors across a serial data set. |
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Keywords: | Multimodality imaging Image registration Brain mapping Brain plasticity Rat brain Imaging |
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