| 血府逐瘀胶囊对单侧输尿管梗阻大鼠Smad信号通路分子的影响 |
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| 引用本文： | 陆敏,周娟,陆海英,王飞,刘煜敏,张悦.血府逐瘀胶囊对单侧输尿管梗阻大鼠Smad信号通路分子的影响[J].时珍国医国药,2008,19(11). |
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| 作者姓名： | 陆敏 周娟 陆海英 王飞 刘煜敏 张悦 |
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| 作者单位： | 1. 上海中医药大学科技实验中心,上海,201203
2. 上海中医药大学医学护理学院,上海,201203 |
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| 基金项目： | 国家自然科学基金(No.30500687);;上海市教育委员会科研项目(No.06cz015);;上海市教育委员会重点学科建设资助项目(No.J50301);; |
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| 摘 要： | 目的探讨血府逐瘀胶囊对单侧输尿管梗阻(unilateral ureteral obstruction,UUO)所致大鼠肾间质纤维化的作用及机理。方法雄性SD大鼠34只随机分为4组:正常组(6只)、假手术组(6只)、模型组(11只)、血府逐瘀胶囊治疗组(11只),采用UUO法建立大鼠肾间质纤维化模型,造模次日予以血府逐瘀胶囊灌胃治疗,3周后检测血清肌酐和尿素氮含量,观察肾功能变化;取肾组织进行HE和六胺银染色(periodic acid-silver metheramine,PASM),观察肾组织病理变化;采用Western blotting分别检测肾组织中磷酸化Smad2(phospho-Smad2,p-Smad2),Smad2,Smad4,Smad7蛋白表达水平,观察Smad信号通路分子表达水平的变化。结果与正常组和假手术组比较,模型组大鼠血肌酐和尿素氮含量显著上升;肾小管基底膜明显增厚,部分肾小管上皮细胞变性,肾小管扩张与萎缩并存,肾间质明显增宽,纤维组织增生,大量炎细胞浸润;p-Smad2和Smad2蛋白表达显著上调,Smad7蛋白表达显著下调(P<0.01),而Smad4蛋白表达水平无明显差异。各项指...
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| 关 键 词： | 血府逐瘀胶囊 肾间质纤维化 Smad通路信号分子 |
Effects of"Xue Fu Zhu Yu Capsule"on Expression of Smad Signal Pathway Molecules in Rats with UUO |
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| LU Min,ZHOU Juan,LU Hai-ying,WANG Fei,LIU Yu-min,ZHANG Yue.Effects of"Xue Fu Zhu Yu Capsule"on Expression of Smad Signal Pathway Molecules in Rats with UUO[J].Lishizhen Medicine and Materia Medica Research,2008,19(11). |
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| Authors: | LU Min ZHOU Juan LU Hai-ying WANG Fei LIU Yu-min ZHANG Yue |
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| Institution: | 1.Scientific Experimental Center of Shanghai University of TCM;Shanghai 201203;China;2.Nursing School;Shanghai University of TCM;China |
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| Abstract: | Objective To study the effect and mechanisms of "Xue Fu Zhu Yu Capsule" on renal interstitial fibrosis induced by unilateral ureteral obstruction in rats.Methods Thirty-four male sprague-dawley rats were randomly divided into four groups: the normal group(n=6),the sham-operated group(n=6),the model group(n=11) and the "Xue Fu Zhu Yu Capsule" treated group(n=11).Model of renal interstitial fibrosisi was established in rats by unilateral ureteral obstruction,and the rats in the treatment group were treated wi... |
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| Keywords: | "Xue Fu Zhu Yu Capsule" Tubulointerstitial fibrosis Smad pathway signal molecules |
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