| 顶青霉木聚糖酶的分离纯化与鉴定 |
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| 引用本文： | 杨瑞金,许时婴,王璋.顶青霉木聚糖酶的分离纯化与鉴定[J].中国脊柱脊髓杂志,1999(2). |
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| 作者姓名： | 杨瑞金 许时婴 王璋 |
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| 作者单位： | 无锡轻工大学食品学院 |
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| 摘 要： | 使用硫酸铵分级沉淀、ＳｅｐｈａｄｅｘＧ－２５凝胶色谱脱盐、ＤＥＡＥ－ＳｅｐｈａｄｅｘＡ－２５和ＣＭ－ＳｅｐｈａｄｅｘＣ－５０离子交换色谱等分离纯化技术，从顶青霉（Ｐｅｎｃｉｌｉｕｍｃｏｒｙｌｏｐｈｉｌｕｍ）培养液中分离到３种木聚糖酶组分，分别称为ＰａｒｔＡ、ＰａｒｔＢ和ＰａｒｔＣ．ＰａｒｔＢ和ＰａｒｔＣ经ＳｅｐｈａｃｒｙｌＳ－２００凝胶过滤色谱和ＣｏｎＡ－Ｓｅｐｈａｒｏｓｅ４Ｂ亲和色谱进一步分离纯化，分别得到２个纯木聚糖酶组分．ＰａｒｔＢ经ＳＤＳ－ＰＡＧＥ鉴定为单带，相对分子质量为２４２００；ＰａｒｔＣ经ＳＤＳ－ＰＡＧＥ鉴定基本为单带，相对分子质量为４８３００
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| 关 键 词： | 顶青霉 木聚糖酶 分离纯化 |
Purification and Identification of Xylanases from Pencillium Corylophilum |
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| YANG Ruijin,XU Shiying WANG Zhang.Purification and Identification of Xylanases from Pencillium Corylophilum[J].Chinese Journal of Spine and Spinal Cord,1999(2). |
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| Authors: | YANG Ruijin XU Shiying WANG Zhang |
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| Abstract: | Three parts of xylanases (Part A, Part B and Part C) were separated and purified from a culture filtrate of Pencillium corylophilum No. P331 by ammonium precipitation, DEAESephadex A25 and CMSephadex C50 column chromatography. Part B was further purifie |
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| Keywords: | xylanase pencillium corylophilum purification |
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