| 还原糖测定方法的规范 |
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| 引用本文： | 管斌,丁友昉,谢来苏,隆言泉. 还原糖测定方法的规范[J]. 中国脊柱脊髓杂志, 1999, 0(3) |
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| 作者姓名： | 管斌 丁友昉 谢来苏 隆言泉 |
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| 作者单位： | 山东轻工业学院!山东济南250100(管斌)，天津轻工业学院!天津300222(丁友昉，谢来苏，隆言泉) |
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| 摘 要： | 探讨了３，５－二硝基水扬酸（ＤＮＳ）添加量、显色时间、氧的溶解量、离子强度以及ＤＮＳ显色后存放时间对纤维素酶酶活力测定的影响．氧的溶解量对测定结果影响显著；测定波长的变化对检测样品光吸收值的灵敏度和线性范围影响很大，一般选定５４０ ｎｍ 为进行纤维素酶酶活力测定的波长．另外，当以葡萄糖为标准来测定还原糖时，不同的测定方法会使得纤维二糖的测定值不同．因此，在测定纤维素酶酶解产物时，用ＤＮＳ方法来测定还原糖，使测定结果更加准确．
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| 关 键 词： | 纤维素酶 酶活力 测定条件 还原糖 3 5-二硝基水扬酸 |
The Modification of the DNS Method for the Determination of Reducing Sugar |
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| GUAN Bin,DING You fang,XIE Lai su,LONG Yan quan. The Modification of the DNS Method for the Determination of Reducing Sugar[J]. Chinese Journal of Spine and Spinal Cord, 1999, 0(3) |
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| Authors: | GUAN Bin DING You fang XIE Lai su LONG Yan quan |
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| Abstract: | In this paper, the procedure and condition (for example, the dosage of DNS reagent, heating time, dissolving oxygen and ionic strength) on the measurement of reducing sugar were studied.Results show that the choice of measuring wavelength was affected by |
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| Keywords: | saccharifying cellulase activity testing condition DNS reducing sugar |
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