| 建立分泌抗人精液抑制素杂交瘤细胞株及其单克隆抗体的研究 |
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| 引用本文： | 徐桂珍,龚守良,刘淑春,张迎春,吕 ,刘树铮,夏风琴.建立分泌抗人精液抑制素杂交瘤细胞株及其单克隆抗体的研究[J].吉林大学学报(医学版),1996(3). |
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| 作者姓名： | 徐桂珍 龚守良 刘淑春 张迎春 吕 刘树铮 夏风琴 |
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| 作者单位： | 吉林省肿瘤研究所 |
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| 摘 要： | 用人精液抑制素免疫ＢＡＬＢ／ｃ小鼠的脾细胞与ＮＳ—１小鼠骨髓瘤细胞融合，获得４株分泌抗人精液抑制素单克隆抗体的杂交瘤细胞株，分别命名为ＩＢ１０，ＩＧ５，ＩＡ４和ＩＡ６。其分泌抗元体均为ＩｇＧ１型，均不与大鼠ＬＨ，ＦＳＨ和ＰＲＬ产生交叉反应，杂交瘤细胞染色体平均为８６～１０３条。细胞经反复冻存、复苏和传代，仍稳定的分泌特异性抗体。
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| 关 键 词： | 人精液抑制素 单克隆抗体 杂交瘤细胞株 |
Preparation of hybridoma cell strains secreting anti-hIB(human semen inhibin)monoclonal antibodies and the study of their McAb |
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| Xu Guizhen,Xia Fengqin,Gong Shouliang.Preparation of hybridoma cell strains secreting anti-hIB(human semen inhibin)monoclonal antibodies and the study of their McAb[J].Journal of Jilin University: Med Ed,1996(3). |
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| Authors: | Xu Guizhen Xia Fengqin Gong Shouliang |
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| Institution: | Department of Radiation Biology;Jilin Resarch Institute of Tumour |
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| Abstract: | The splencytes of BALB/c mice immunized with human semen inhibin(hIB)wereused to fuse with the myeloma cells of NS 1 mice,Four clones secreting anti-hIB McAb were ob-tained.They were named IB10,IG5,IA4 and IA6,Rospectively. All anti-hIB McAbs secreted from theclones were lgG1 subclass,and immunological cross reaction was not produced with rat LH,FSH andPRL,The mean of chromosmes of hybridoma cells was 86 103.After repeatedly frozen,thawed andpassaged,the four hybridoma cells were proved to be capable of secreting specific McAb stably. |
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| Keywords: | human semen inhibin McAb hybridoma cell strains |
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