Therapeutic potential of dental pulp stem cell transplantation in a rat model of Alzheimer’s disease |
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Authors: | Xue-Mei Zhang Yuan-Jiao Ouyang Bing-Qian Yu Wei Li Mei-Yu Yu Jin-Yue Li Zhuo-Min Jiao Dan Yang Na Li Ying Shi Yun-Yun Xu Zhi-Jun He Duo Wang Hui Yue Jin Fu |
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Affiliation: | 1.Department of Neurology, The 2nd Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China;2.Department of Neurology, Ningbo Hangzhou Bay Hospital, Ningbo, Zhejiang Province, China;3.Department of Neurology, the First Hospital of Qiqihar, Qiqihar, Heilongjiang Province, China |
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Abstract: | Dental pulp stem cells are dental pulp-derived mesenchymal stem cells that originate from the neural crest. They exhibit greater potential for the treatment of nervous system diseases than other types of stem cells because of their neurogenic differentiation capability and their ability to secrete multiple neurotrophic factors. Few studies have reported Alzheimer’s disease treatment using dental pulp stem cells. Rat models of Alzheimer’s disease were established by injecting amyloid-β1–42 into the hippocampus. Fourteen days later, 5 × 106 dental pulp stem cells were injected into the hippocampus. Immunohistochemistry and western blot assays showed that dental pulp stem cell transplantation increased the expression of neuron-related doublecortin, NeuN, and neurofilament 200 in the hippocampus, while the expression of amyloid-β was decreased. Moreover, cognitive and behavioral abilities were improved. These findings indicate that dental pulp stem cell transplantation in rats can improve cognitive function by regulating the secretion of neuron-related proteins, which indicates a potential therapeutic effect for Alzheimer’s disease. This study was approved by the Animal Ethics Committee of Harbin Medical University, China (approval No. KY2017-132) on February 21, 2017.Chinese Library Classification No. R456; R741; Q2 |
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Keywords: | Alzheimer''s disease brain central nervous system dental pulp stem cell in vivo model rat stem cells transplantation |
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