| 气相色谱－质谱联用法测定7－甲基鸟嘌呤 |
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| 引用本文： | 刘丽娜,李阳,张达生,谢音. 气相色谱－质谱联用法测定7－甲基鸟嘌呤[J]. 武汉大学学报(医学版), 1994, 0(4) |
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| 作者姓名： | 刘丽娜 李阳 张达生 谢音 |
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| 作者单位： | Department of Chemistry，Hubei Medical University，Wuhan 430071，China |
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| 摘 要： | 利用气相色谱－质谱联用仪测定ＤＮＡ烷基化产物７－甲基鸟嘌呤，在本文所介绍的测定条件下，用Ｎ－甲基－Ｎ－（三甲基硅烷基）三氟乙酰胺使７－甲基鸟嘌呤形成稳定的硅烷化衍生物，该衍生物可电离成（Ｍ－ＣＨ３）＋碎片离子，特征峰明显，有利于定量及结构分析，测定灵敏度可达ｎｇ级。
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| 关 键 词： | 质量碎片质谱法；鸟嘌呤／类似物和衍生物；鸟嘌呤／分离和提纯 |
Determination of 7-Methylguanine by Gas Chromatography-Mass Spectrometry |
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| Liu Lina,LI Yang,Zhang Dasheng,et al. Determination of 7-Methylguanine by Gas Chromatography-Mass Spectrometry[J]. Medical Journal of Wuhan University, 1994, 0(4) |
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| Authors: | Liu Lina LI Yang Zhang Dasheng et al |
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| Abstract: | With the method of determining 7-methylguanine with gas chromatography-mass spectrometry was introduced, silicify derivation can be made from 7-methylguanine with N-methyl-N-(trimethylsilyl)-trifluoracetamide (MSTEA).Under the determined conditions introduced in the thesis ,the derivation would be ionized and fragmented into massive minus 15 fragments(M-CH_3) the specific peak is quite clear and easily be analysised quantitatively and structucturally. |
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| Keywords: | mass fragmentography spectrum analysis mass guanine/AA guanine/IP |
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