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| 细胞遗传学联合免疫细胞化学检查在首诊非霍奇金淋巴瘤骨髓侵犯中的应用 | |
| 引用本文： | 王娜,谢守军,申兴斌,武英伟,宗春光.细胞遗传学联合免疫细胞化学检查在首诊非霍奇金淋巴瘤骨髓侵犯中的应用[J].中国实验诊断学,2010,14(4):582-584. |
| 作者姓名： | 王娜 谢守军 申兴斌 武英伟 宗春光 |
| 作者单位： | 1. 承德医学院附属医院,ICU,河北,承德,067000 2. 承德医学院附属医院,检验科 3. 承德医学院附属医院,病理科 |
| 摘 要： | 目的探讨细胞遗传学和免疫细胞化学检查在非霍奇金淋巴瘤骨髓侵犯(NHL-BMI)中的诊断价值。方法骨髓标本直接涂片Wright-Gimsa染色观察其形态学改变。应用骨髓细胞短期培养法制备染色体标本,以R显带技术进行核型分析。分离骨髓单个核细胞进行免疫细胞化学染色。结果24例淋巴瘤患者,骨髓受累程度各异,淋巴瘤细胞形态多样。CD20和CD79a对B细胞来源、CD3对T细胞来源淋巴瘤有诊断价值。有染色体核型异常的病例占54%(13/24),2例骨髓轻度受累患者检出核型异常。结论综合细胞遗传学、免疫细胞化学和细胞形态学检测,有助于NHL骨髓侵润的诊断,特别是对无表浅淋巴结肿大的NHL患者可能提供诊断依据。 |
| 关 键 词： | 非霍奇金淋巴瘤 细胞遗传学 免疫细胞化学 细胞形态学 |
Evaluation of the combined examination with cytogenetic and immunocytochemistry in non-hodgkin lymphoma bone marrow involvement at initial diagnosis |
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| WANG Na,XIE Shou-jun,SHENG Xing-bin,et al..Evaluation of the combined examination with cytogenetic and immunocytochemistry in non-hodgkin lymphoma bone marrow involvement at initial diagnosis[J].Chinese Journal of Laboratory Diagnosis,2010,14(4):582-584. | |
| Authors: | WANG Na XIE Shou-jun SHENG Xing-bin |
| Institution: | WANG Na,XIE Shou-jun,SHENG Xing-bin,et al.(The Hospital Affiliated to Chengde Medical College,Chengde 067000,China) |
| Abstract: | Objective To assess the value of cytogenetic and immunocytochemistry examination in non-Hodgkin lymphoma bone marrow involvement(NHL-BMI)at initial diagnosis.Methods Morphological changes of bone marrow smears stained with Wright-Gimsa were observed under microscope.Chromosome specimens were prepared by short-term culture of bone marrow cells and karyotype Analyses were carried out using R-banding techniques.Unidentified and uninuclear cell isolated from bone marrow were studied by using immunocytochemical ... |
| Keywords: | non-hodgkin lymphoma cytogenetic immunocytochemistry morphology |
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