Low noise,self-phase-modulation-enabled femtosecond fiber sources tunable in 740-1236 nm for wide two-photon fluorescence microscopy applications |
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| Authors: | Lu-Ting Chou Yu-Cheng Liu Dong-Lin Zhong Wei-Zhong Lin Hao-Hsuan Hung Chao-Jin Chan Zi-Ping Chen Shih-Hsuan Chia |
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| Affiliation: | 1.Institute of Biophotonics, National Yang-Ming University, No. 155, Sec. 2, Linong Street, Taipei 11221, Taiwan;2.Institute of Biophotonics, National Yang Ming Chiao Tung University, No. 155, Sec. 2, Linong Street, Taipei 11221, Taiwan |
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| Abstract: | We have demonstrated widely tunable Yb:fiber-based laser sources, aiming to replace Ti:sapphire lasers for the nJ-level ultrafast applications, especially for the uses of nonlinear light microscopy. We investigated the influence of different input parameters to obtain an expansive spectral broadening, enabled by self-phase modulation and further reshaped by self-steepening, in the normal dispersion regime before the fiber damage. We also discussed the compressibility and intensity fluctuations of the demonstrated pulses, to reach the transform-limited duration with a very low intensity noise. Most importantly, we have demonstrated clear two-photon fluorescence images from UV-absorbing fluorophores to deep red dye stains. |
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