Fas基因转染对舌鳞癌细胞系Tca8113凋亡的影响

目的探讨外源性Fas基因介导舌鳞癌细胞凋亡的可行性及其可能的分子机制。方法脂质体法将重组人Fas真核表达载体转染人舌鳞癌细胞系Tca8113，抗Fas单克隆抗体诱导其凋亡，Western blotting检测转染前后Tca8113细胞Fas蛋白表达水平，TUNEL法检测转染前后细胞凋亡水平。结果转染后Tca8113细胞Fas蛋白表达上调；抗Fas单克隆抗体可诱导Tca8113细胞凋亡，凋亡指数升高。结论上调Fas基因表达及应用抗Fas单克隆抗体可诱导肿瘤细胞凋亡，有可能作为肿瘤基因治疗的新途径。

Study on the Apoptosis Mediated by Fas Gene Transfer in Tca8113 Cell Line

Objective To investigate the possibility and possible mechanism of apoptosis in tongue squamous cell carcinoma mediated by exogenie Fas gene transfer. Methods Eukaryotic expressing plasmid BK-Fas was reconstructed and transferred into human tongue squamous cell eareinoma cell line Tca8113 by lipofeetamine; anti-Fas monoelonal antibody was used to induee Tca8113 apoptasis, Western blotting assay was applied to detect the expression of Fas protein pre- and post transfection; TUNEL was applied to examine the change of tumor cell apoptasis. Results Up-regulations of F as protein expression were found in Fas transfected eels. Anti-Fas monoelonal antibody could be used in the induction of tumor cell apoptasis. Conclusion Up-regulation of Fas expression can be a new pathway of tumor gene therapy to mediate tumor cell apoptosis.