亚硒酸钠溶液诱导胃上皮肿瘤SGC-7901细胞凋亡的实验研究

目的：探讨亚硒酸钠能否诱导胃上皮肿瘤SGC 7901细胞凋亡，以及凋亡诱导与细胞周期改变的关系。方法：亚硒酸钠溶液不同浓度、不同作用时间分别处理SGC 7901细胞，应用电子显微镜观察亚硒酸钠溶液作用后细胞形态变化，应用DNA末端原位标记染色法 (TUNEL)及流式细胞仪技术分析细胞凋亡及细胞周期的改变。结果：亚硒酸钠作用于SGC 7901细胞后，可看到较为典型的细胞凋亡的形态学变化：细胞核固缩、染色质凝集、呈新月型紧贴于核膜周边，核碎裂，染色质片段化，凋亡小体形成等。流式细胞仪DNA直方图上出现典型的亚二倍体的“凋亡峰”。TUNEL染色法细胞凋亡指数在8.4％～33.4％。结论：亚硒酸钠能够诱导SGC 7901细胞凋亡，亚硒酸钠诱导SGC 7901细胞凋亡与细胞周期阻滞密切相关。

Apoptosis of gastric epithelial tumor SGC-7901 cells by sodium selenite

Objective: To investigate whether sodium selenite can efficiently induce SGC-7901 cells,and to study the relationship between the apoptosis and the cell cycle.Methods: Being treated with sodium selenite under different concentrations and different times,SGC-7901 cells were observed the morphologic changes by electron microscope,and the apoptosis and the cell cycle by TUNEL method and flow cytometry.Results: After 12 hours of exposure to the drug,changes like cell shrinkage,nuclear condensation and formation of apoptotic bodies were exhibited.A typical sub-diploid peak before G_0/G_1 phase was observed by flow cytometry.The apoptosis index was 8.4% to 33.4% by TUNLE.Conclusion: Sodium selenite can induce the apoptosis of SGC-7901 cells,which is associated with the blockage of cell cycle.