2型糖尿病大鼠胰腺中Homer的表达及意义 |
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引用本文: | 许淑芳,邹辉,吴三云,涂建成. 2型糖尿病大鼠胰腺中Homer的表达及意义[J]. 基础医学与临床, 2013, 33(7): 868-872 |
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作者姓名: | 许淑芳 邹辉 吴三云 涂建成 |
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作者单位: | 1. 武汉大学中南医院检验科基因诊断中心,湖北武汉,430071 2. 湖北省黄冈市中心医院内分泌科,湖北黄冈,438000 |
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基金项目: | 基金项目:973计划项目《基于核酸的重大疾病诊断新策略和新技术研究》 |
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摘 要: | 目的 探讨Homer与胰岛素分泌及2型糖尿病发病机制的相关意义.方法 建立2型糖尿病大鼠模型,检测血糖和血胰岛素值.取胰腺组织,采用反转录PCR、免疫组化和免疫荧光方法检测Homer(Homer1c、Homer2、Homer3)和胰岛素的表达.结果 大鼠胰腺组织可见Homer表达,糖尿病大鼠胰腺组织胰岛素表达明显减少(P<0.01),而Homer1c、Homer2和Homer3均表达增加(P<0.05).结论 Homer基因在大鼠胰腺组织亦有表达,Homer1c、Homer2和Homer3可能对胰岛素分泌起负性调控作用.
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关 键 词: | 2型糖尿病 胰腺 Homer 表达 |
Homer expression in the pancreases of type 2 Diabetes mellitus rats |
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Abstract: | Objective To study the expression and correlation of Homer and insulin in rats with Type 2 Diabetes mellitus. Methods 22 Diabetes mellitus rats and 15 normal rats were sacrificed, the pancreases of rats were taken to detect Homer (Homerlc, Homer2 and Homer3) and insulin by using Polymerase Chain Reaction (PCR), Immunohistochemistry and double immunofluorescent labeling methods. Results Homerl, Homer2 and Homer3 were positive in the pancreases. In rats with Diabetes mellitus, leverages of insulin were much lower, but expressions of Homer1c, Homer2 and Homer3 protein were much higher. Conclusion The result shows that there is a significant change in the expression of Homer1c, Homer2,Homer3 and insulin between two groups.Homer may play important roles in insulin secretion,Homer1c, Homer2,Homer3 may act as inhibitors. |
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Keywords: | Type 2 diabetes mellitus pancreatic islets Homer expression |
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