SD大鼠脊髓损伤前后外周血T淋巴细胞亚群的变化

目的:探讨SD大鼠脊髓损伤(spinal cord injury,SCI)前后外周血T淋巴细胞亚群的动态变化。方法:取22只健康SD大鼠(雌雄各半),采用NYU脊髓损伤仪制作重物坠落SCI模型。于SCI前、SCI后1、3、5、7和14 d采外周血,用流式细胞术检测外周血淋巴细胞中CD3+T、CD3+CD4+T和CD3+CD8+T细胞比例的变化。结果:SCI前与SCI后1、7和14 d CD3+CD8+T细胞比例均无明显变化(P0.05),而SCI后第3、5 d均高于SCI前14 d(P0.01)。SCI后1、3、5 d及7 d的CD3+T细胞、CD3+CD4+T细胞比例,以及CD3+CD4+/CD3+CD8+比值均明显低于SCI前(P0.01)。在SCI后14 d,上述各T细胞亚群的比例均恢复到术前水平,两时间点之间比较差异均无统计学意义(P0.05)。结论:SCI后,SD大鼠总T细胞和CD4+T细胞明显降低,CD3+CD4+/CD3+CD8+下降。提示SCI后的早期阶段,可能存在T细胞功能的异常。

The change of T lymphocyte subsets in peripheral blood of SD rats with acute spinal cord injury

Objective:To investigate the change of T lymphocyte subsets in peripheral blood of SD rats with acute spinal cord injury(SCI).Methods:Twenty two SD rats(11 each male and female) were used to establish SCI model using NYU Spinal Cord Contusion Impactor System.The CD3 +,CD3 + CD4 + and CD3 +,CD8 + T cells,and the proportion of CD3 + CD4 + to CD3+ CD8+ T cells of peripheral blood of all rats were evaluated using flow cytometry at the time points of preinjury and days 1,3,5,7and 14 postinjury.Results:The percentages of CD3+ CD8+ cells had no significant difference among before SCI,the 1st,7th and 14th day after SCI,butthe percentage of CD3+,CD8+ T cells at the 3rd,5th day was higher than at the 14th day after SCT and before SCI.The percentages of CD3+ and CD3+ CD4+ T cells,and ratio of CD3+ CD4+ to CD3+ CD8+ T cells at days 1,3,5 and 7 post-injury were significantly lowerthan those of at preinjury(P < 0.01).After 14 days postinjury,all T lymphocyte subsets were restored to preoperative level,which wereno differences with preinjury(P > 0.05).Conclusions:After acute SCI,the total of T cells and CD4+ T cells and the ratio of CD3+ CD4+ to CD3+ CD8+ T cells decrease,which suggest there may be T cell function abnormalities in the early stage of SCI in rat model.