人乳头状瘤病毒和EB病毒在子宫颈上皮内瘤变中的表达

目的:探讨人乳头状瘤病毒(HPV)和EB病毒(EBV)在子宫颈上皮内瘤变(CIN)中的表达.方法:采用原位杂交对130例CIN(CIN1 50例,CIN2 40例,CIN3 40例)、子宫颈癌10例、子宫颈湿疣样病变10例、子宫颈正常黏膜10例的HPV(高危型16/18、低危型6/11)和EBV进行检测.结果:HPV16/18在CIN1、CIN2、CIN3的阳性表达率分别是44.0%、37.5%和62.5%,子宫颈癌为8/10,子宫颈湿疣样病变为10/10,子宫颈正常黏膜为3/10,差异有统计学意义(P<0.01).HPV6/11在CIN1、CIN 2、CIN3的表达率分别是36.0%、52.5%和70.0%,在子宫颈癌中的表达是6/10,子宫颈湿疣样病变9/10,子宫颈正常黏膜3/10,差异有统计学意义(P<0.01).HPV16/18和HPV6/11在CIN1、CIN2、CIN3中联合表达率分别为26.0%、22.5%和50.0%,子宫颈癌为6/10,子宫颈湿疣样病变为7/10,子宫颈正常黏膜为2/10,差异有统计学意义(P<0.01).EBV在CIN1、CIN2、CIN3的阳性表达率分别是24.0%、12.5%和22.5%,子宫颈癌为3/10,子宫颈湿疣样病变为0/10,子宫颈正常黏膜为0/10,差异无统计学意义(P>0.05).结论:HPV感染是引起CIN的主要致病因子,临床上往往是多种亚型混合感染.EB病毒在CIN和子宫颈癌表达的意义仍有争议.

Expression of human papillomavirus and Epstein-Barr virus in cervical intraepithelial neoplasia

Objective:To explore the expression of human papillomavirus(HPV) and Epstein-Barr virus(EBV) in cervical intraepithelial neoplasia(CIN).Methods:The high-risk HPV(16/18),low-risk HPV(6/11) and EBV of 130 cases of CIN(50 CIN1,40 CIN2,40 CIN3),10 cases of invasive cervical carcinomas,10 cervical condyloma-like lesions and 10 normal mucosa of the uterine cervix were detected using in situ hybridization(ISH) technique.Results:The positive expression rates of HPV16/18 in CIN1,CIN2 and CIN3 were 44.0%,37.5% and 62.5%,respectively;and positive HPV16/18 was detected in 8/10 of the invasive cervical carcinomas,10/10 of cervical condyloma-like lesions and 3/10 of normal mucosa of the uterine cervix(P<0.01).The positive expression rates of HPV6/11 in CIN1,CIN2 and CIN3 were 36.0%,52.5% and 70.0%,respectively;and positive HPV6/11 was detected in 6/10 of the invasive cervical carcinomas,9/10 of cervical condyloma-like lesions and 3/10 of normal mucosa of the uterine cervix(P<0.01).The combined positive expression rates of HPV16/18 and HPV6/11 in CIN1,CIN2 and CIN3 were 26.0%,22.5%,and 50.0%,respectively;and combined positive expression of HPV16/18 and HPV6/11 was detected in 6/10 of the invasive cervical carcinomas,7/10 of cervical condyloma-like lesions and 2/10 of normal mucosa of the uterine cervix(P<0.01).The positive expression rates of EBV in CIN1,CIN2 and CIN3 were 24.0%,12.5% and 22.5%,respectively;and EBV was detected positive in 3/10 of the invasive cervical carcinomas,0/10 of cervical condyloma-like lesions and 0/10 of normal mucosa of the uterine cervix(P>0.05).Conclusions:HPV infection is the major etiological factor for CIN,clinically often coinfected with some variants.The signification of positive expression of EBV in CINs and invasive cervical carcinomas is still debatable.