HFRS患者血浆中TNF、sIL-2R、IL-6、IL-4和IFN-γ水平的变化及其意义

目的 :探讨肾综合征出血热 (HFRS)患者血浆中的TNF、sIL 2R、IL 6、IL 4和IFN γ水平的变化及其与血清中丙氨酸转氨酶ALT活性水平的相关性。方法 :利用双mAb夹心ELISA法检测HFRS患者血浆中细胞因子的水平 ,应用美国RA 10 0 0全自动生化仪检测患者血清中ALT的水平。结果 :HFRS患者血浆中TNF、IL 6、IL 4、IFN γ和sIL 2R水平分别为 (95 .82± 12 .0 4 )、(36 2 .4 6± 14 1.2 6 )、(17.76± 3.5 2 )、(116 .18± 19.80 )ng/L及 (89882 0± 12 72 0 0 )U/L ,健康对照组依次为 (17.89± 1.6 8)、(4 3.81± 18.0 8)、(4 .86± 1.14 )、(7.5 7± 2 .4 1)ng/L及(6 6 730± 2 96 90 )U/L、(P <0 .0 1) ;患者血清中ALT的水平也显著升高 ,为正常对照的 4 .4倍。通过相关性分析 ,发现TNF、sIL 2R、IL 6和IFN γ水平与患者血清中ALT的水平高度相关 (P <0 .0 1)。结论 :HFRS患者体内TNF、sIL 2R、IL 6和IFN γ水平显著升高 ,且与患者体内ALT水平的升高高度相关 ,提示HTNV感染所致肝脏的损伤可能与上述细胞因子水平的升高有关

Changes and significance of TNF,sIL-2R,IL-6,IL- 4 and IFN-γ levels in plasma from the patients with hemorrhagic fever with renal syndrome

AIM: To explore the changes of TNF, sIL-2R, IL-6, IL-4 and IFN-gamma levels in plasma from patients with hemorrhagic fever with renal syndrome(HFRS) and analyze the correlation between these cytokine levels and alanine aminotransferase(ALT) level in sera from the patients. METHODS: The cytokine levels were measured by double mAb sandwich ELISA. An automatic biochemical detector(RA-1000) was employed to determine the ALT level. RESULTS: Levels of TNF, IL-6, IL-4, IFN-gamma and sIL-2R in plasma from the patients were(95.82+/-12.04), (362.46+/-141.26), (17.76+/-3.52), (116.18+/-19.80) ng/L and (898 820+/-127 200) U/L, respectively. These cytokines in plasma from healthy donors were (17.89+/-1.68), (43.81+/-18.08), (4.86+/-1.14), (7.57+/-2.41) ng/L and (66 730+/-29 690) U/L, respectively. The cytokine levels of the patients were notably higher than those of the healthy donors.(P<0.01). At the same time, the average ALT level in sera from the patients was 4.4 fold of that from the healthy donors. Statistical analysis showed that levels of TNF, sIL-2R, IL-6 and IFN-gamma were positively correlated with ALT level, respectively.CONCLUSION: The HFRS patients have a significant increase in both cytokines (TNF, sIL-2R, IL-6 and IFN-gamma) and ALT levels. And there is a positive correlation between those cytokine and ALT levels, suggesting that the liver injury during HTNV infection may be related to the elevation of above cytokine levels.