[125I]protein a solid-phase assay for detection of monoclonal antibody |
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Authors: | Florence J. Nicholson |
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Affiliation: | (1) Hybridoma Technology Services, Hazleton Biotechnologies Corporation, Inc., 9200 Leesburg Turnpike, 22180 Vienna, Virginia, USA |
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Abstract: | Summary The production of monoclonal antibodies involves testing hundreds of hybridoma supernatant samples for antibody content. This necessitates a rapid detection assay which can separate antibody of interest from antibodies that react nonspecifically. Solid-phase radioimmunoassay is one such method. |
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Keywords: | solid-phase radioimmunoassay monoclonal antibody Protein A |
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