| 正交试验法优选小鼠四氯化碳矸损伤模型 |
| |
| 引用本文： | 赵艳玲,蔡光明,刘军,张新全,肖小河,袁海龙,贺承山. 正交试验法优选小鼠四氯化碳矸损伤模型[J]. 解放军药学学报, 2001, 17(4): 215-217 |
| |
| 作者姓名： | 赵艳玲 蔡光明 刘军 张新全 肖小河 袁海龙 贺承山 |
| |
| 作者单位： | 中国人民解放军302医院药学部 |
| |
| 摘 要： | 目的；采用正交试验法优选小鼠四氯化碳肝损伤最佳模型制作条件，方法：选择对小鼠血清转氨酶（ALT）有影响的四个因素（1）四氯化碳浓度；（2）肝毒剂中毒剂量；（3）中毒次数；（4）中毒途径，生个因素取3个水平，按L9(3^4)正交试验表安排是试验，以阳性药联苯双酯双小鼠四氯化碳肝损伤改善率为考察指标，评定四氯化碳中毒方案的优劣，结果：采用0．1%(v/v)的四氯化碳，按每10g小鼠0.2ml剂量腹腔一次注射的给药方案为佳，结论 以正交试验法优选出的小鼠四氯化碳肝损伤模型制作方案是可行的。
|
| 关 键 词： | 正交试验 四氯化碳 肝损伤 模型 |
| 文章编号： | 1008-9926(2001)04-0215-03 |
| 修稿时间： | 2000-12-12 |
Optimum Method Selection of CCl_4 Injured Liver Model in Mice by Orthogonal Test |
| |
| ZHAO Yan Ling,CAI Guang Ming,LIU Jun,ZHANG Xin Quan. Optimum Method Selection of CCl_4 Injured Liver Model in Mice by Orthogonal Test[J]. Pharmaceutical Journal of Chinese People's Liberation Army, 2001, 17(4): 215-217 |
| |
| Authors: | ZHAO Yan Ling CAI Guang Ming LIU Jun ZHANG Xin Quan |
| |
| Abstract: | Aim To explore the optimum method of CCl 4 injured liver model in mice by orthogonal test.Methods Four influential factors on transaminase in mice serum were chosen:(1)Concentration of CCl 4;(2)Dose of liver toxicosis;(3)Times of toxicosis;(4)Approaches of toxicosis,three levels of every factor.Arranged the tests based on L 9(3 4) orthogonal test table,improved ratio of Biphenyl dimethyl dicarboxylate (DDB) Tablet on CCl 4 injured liver in mice was regarded as detecting index,the optimum method of CCl 4 toxicosis was assessed.Results The optimum method was that:0.1%(v/v) CCl 4,0.2ml every 10g mice,ip,1time.Conclusion The injured liver model by CCl 4 selected by orthogonal test was feasible. |
| |
| Keywords: | Orthogonal test CCl 4 Liver injury Model |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
