桑叶总酚成分的提取纯化及成分定性

目的:研究桑叶总酚类化合物的提取纯化及成分鉴定,为桑叶总酚的降血糖作用机制研究提供理论依据。方法:以芦丁提取率为考察指标,通过正交实验确定最佳乙醇提取工艺,优选纯化工艺路线及大孔树脂纯化工艺的技术参数,包括树脂用量,pH,洗脱剂浓度等,并通过UPLC-MS对总酚部位中主要化学成分进行分析鉴定。结果:最佳提取工艺为加入10,8倍量70%乙醇,每次提取1.5h。采用两次大孔树脂纯化,第一次用1%Na2CO3洗脱,第二次用60%乙醇洗脱。通过UPLCMS鉴定出8个酚类化合物。结论:该工艺简单,周期短,成本低,成分含量大于50%,达到了有效部位的要求,适合工业化生产,该方法可为开展桑叶总酚的降血糖作用研究提供理想的实验药物。

Purification and Qualitative Analysis of Total Phenolics from Morus alba Leaves

Objective: The extraction and purification methods for phenolics in Morus alba leaves were studied and the constituents of phenolic were identified, provided suitable experimental drug specimen for exploring the hypoglycemic effect of M. alba leaves. Method: The optimum extraction condition was investigated by orthogonal design and the extraction quantity was regarded as the investigated index. The factors affecting separation, such as resin dosage, pH and concentration of eluting agent were considerate. The chemical components of phenolic extracted from M. alba were analyzed and identified by UPLC-MS. Result: The optimized extracting conditions were as follows:extracted two times with 70% alcohol, each 1.5 h. The ratio of solvent to material 10:1 and 8:1, respectively;and the purification process with macroporous resin HPD-826 was using 1%Na₂CO₃, 60% ethanol to wash out phenolics;eight phenolics compounds were identified by UPLC-MS. Conclusion: This method is simple, short cycle, low cost, high content and suitable for industrialized production. This purification technology could be used to extract phenolics of M. alba leaves for studies on its hypoglycemic effect.