Protection of sivelestat sodium in ischemia reperfusion brain injury in rats

Objective： To explore the protective effect and its mechanism of sivelestat sodium （SS） on cerebral ischemia reperfusion in rats. Methods： The neurons of neonate rat were cultured in vitro. The protective effects of sivelestat sodium on ischemic injury were observed by treating cells in glucose - free and oxygen -free medium. Neuron survival was measured by MTT reduction assay and the release of LDH; neutrophile elastase contents were determined by ELISA assay; intracellular calcium levers were measured by fluo -3 and confocal laser microscopy; malondialdehyde （MDA） contents, superoxide dismutase （SOD） activity and glutamic acid contents in neuron were measured by colorimetric method. The expression of Bcl - 2 and Bax in rat neurons were detected by immunohistochemistry ; the mrna sequences of Bcl - 2 and Bax were detected by RT - PCR; the expression of Bcl - 2, Bax and p - p38 MAPK proteins in rat neurons was detectd by Western blotting. Results： Sivelestat sodium protected the integrity and survival of cells and reduced LDH leakage, MDA contents, ncutrophile elastase contents, glutamic acid contents; increased SOD contents; decreased the mrna expression of bax and increased the mrna expression of Bcl - 2 ; decreased the protein expression of p - p38MAPK . The ratio of Bcl - 2/Bax in hippocampus neurons of models was lower than that of Sivelestat sodium groups. Conclusions： SS may play neuroprotectvie role by increasing neuron survival, reducing oxygen free radicals, calcium release, glutamate release and the expressions of bax and p -p38MAPK, and down - regulating the expressions of bcl -2.

Protection of sivelestat sodium in ischemia reperfusion brain injury in rats