不同频率电针对大鼠杏仁核内生长抑素mRNA表达的影响

目的:观察不同频率电针大鼠“足三里”穴对杏仁核内生长抑素(SOM)mRNA表达的影响。方法:雄性SD大鼠30只,随机分为对照组2、Hz电针组和128 Hz电针组,每组10只。采用原位分子杂交方法,观察不同频率电针大鼠一侧“足三里”穴30 min后,大鼠杏仁核内SOM mRNA的表达。结果:电针对大鼠杏仁核内SOM mRNA的表达有上调作用,并且高频电针作用强于低频电针。电针后杏仁核各亚细胞群内SOM mRNA阳性神经元的数量增加以中、小型细胞较为明显;在细胞染色程度上以中等程度染色和淡染细胞的数量增加为主,细胞平均灰度值有所增加。结论:不同频率电针对大鼠杏仁核内SOM mRNA的表达有明显影响。

Effect of Different Frequencies of Electroacupuncture on Somatostatin mRNA Expression in Rats' Amygdaloid Nucleus

Objective:To observe the effect of different frequencies of electroacupuncture(EA) of "Zusanli"(ST 36) on the expression of somatostatin(SOM) mRNA in rats' amygdaloid nucleus.Methods: Thirty SD rats were randomized into control,2 Hz-EA and 128 HzEA groups with 10 cases in each group.Left "Zusanli"(ST 36) was punctured and stimulated electrically for 30 min by setting the parameters being 2 Hz and 128 Hz in frequency,3 V in strength,and 1 ms in the duration of waves.After EA, the rats were transcardically perfused with 1% paraformaldehyde and 1.25% glutaraldehyde and 0.1 mol/L phosphate buffer solution.The brain tissues were sampled to cut into sections(40 μm).The expression of SOM mRNA of amygdaloid nucleus was detected by using in situ hybridization technique.Results: Compared with control group,the number of SOM mRNA expression positive neurons and the grey-density values of SOM mRNA expression in amygdaloid nucleus on the bilateral sides increased significantly in 2 Hz and 128 Hz EA groups(P<0.05,0.01).There were significant differences between two EA groups in the number of SOM mRNA expression positive neurons and the grey-density values.Conclusion: Both 2 Hz and 128 Hz EA of "Zusanli" can up-regulate the expression of SOM mRNA in the rat's amygdaloid nucleus.The high frequency of EA has a more obvious effect.