中性粒细胞在大鼠深Ⅱ度烧伤创面早期进行性损伤中的作用

目的 探讨中性粒细胞(PMNs)在大鼠深Ⅱ度烧伤创面早期进行性损伤中的作用。方法采用SD大鼠深Ⅱ度烫伤模型，检测烧伤后大鼠外周血PMNs数量、创面组织中髓过氧化物酶(MPO)活性、丙二醛(MDA)含量，Masson’s三色染色及抗Vimentin免疫组化染色显示创面坏死组织。结果 外周血PMNs伤后即刻增加，4h达高峰；创面组织MPO活性伤后24h达高峰；MDA含量伤后0．5h达最低，至48h才高于正常；组织学观察伤后48h坏死组织量显多于伤后24h。结论 PMNs导致的创面损伤集中发生在伤后24h后。提示阻断PMNs粘附以减轻深Ⅱ度烧伤创面早期进行性损伤，需在伤后4h内进行，而使用抗氧化剂则应在伤后24h内进行。

The role of PMNs ga early progressive injury of deep partial-thickness burn wound

Objective To examine the role of polymophomuclear neutophils (PMNs) in early progressive injury of deep partial-thickness burn wound. Methods After deep partial-thickness burn, the count of PMNs in beripheral circulation, activity of myeloperoxidase (MPO) and content of MDA in wound tissue of Spaque Dawley rats, were measured. Sections of wound biopsies were stained with Masson's thrichromatic staining and anti-vimentin immunohistopatholog- ic staining for identifying the injured tissue. Results PMNs counts increased immediately and reached a peak at 4 h postburn, and MPO activity Peaked at 24 h postburn. MDA content was lowe at 0.5 h and higher at 48 h postburn than that of normal control. Histologic analysis showed that there was more necrotic tissue at 48 hr postburn than that at 24h. Conclu- sion It was after 24h postburn that PMNs induced tissue damage. The results suggest that intervention of PMNs adhe- sion should be under taken in about 4 h after thermal injury and anti-oxidizing agent should be given before 24 h postburn in order to prevent early progressive injury to the wound.