血管平滑肌细胞体外摄取寡核苷酸探针的研究

目的:以99mTc标记针对c-myc和bcl-2 mRNA的寡核苷酸并检测其在血管平滑肌细胞(VSMC)中的摄取情况,初步探讨其在粥样硬化病变中的诊断价值。方法99mTc标记寡核苷酸后分别加入体外培养对数生长期及平台期猪冠状动脉VSMC,37℃条件下温育,检测不同时段摄取百分数。结果:增殖期或平台期VSMC对bcl-2探针的摄取均低于对c-myc探针的摄取。在c-myc探针组,增殖期细胞较平台期摄取高反义探针较正义探针摄取高。结论:增殖期VSMC对c-myc反义探针的摄取增高和(或)对bcl-2反义探针的摄取减少,有望成为粥样病变的早期诊断指标。

Technetium-99m Labeled Oligonucleotides Uptake in Vascular Smooth Muscle Cells

Objective: To investigate the smooth muscle cells(SMCs) uptake of 99m Tc- labeled c-myc or bcl-2 oligonucleotides. Method: The oligonucleotides(A-SON or SON) were conjugated with S-Acetyl-MAG3-NHS and labeled with 99mTc. 99mTc-MAG3-DNA radioactivity incorporation into porcine coronary SMCs in the log and plateau phase, respectively, was determined; in vitro after different times of incubation at 37℃ . Results: The SMC shows higher uptake for c-myc probe than bcl-2 probe. The normalized uptake per million cells is higher for the antisense than for the sense c-myc probe, it is also higher for the SMC in log rather than in plateau phase. Percentage uptake of bcl-2 antisense probe by the SMC is slightly lower in log phase than in plateau phase Conclusion: These data suggest that 99m Tc-MAG3-DNA should be considered further as an early specifictraceer in noninvasive diagnosis of arterosclerotic lesions. Increased uptake of c-myc antisense probe accompanied with diminished uptake of bcl-2 antisense probe may suggest a lesion of atherosclerosis at an early stage.