| 雷公藤多糖的提取和含量测定方法研究 |
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| 引用本文： | 梁惠花,刘晓河,王志宝,郭春燕. 雷公藤多糖的提取和含量测定方法研究[J]. 中国现代应用药学, 2004, 21(1): 8-10 |
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| 作者姓名： | 梁惠花 刘晓河 王志宝 郭春燕 |
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| 作者单位： | 张家口医学院,河北,张家口,075000 |
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| 摘 要： | 目的建立雷公藤中多糖的测定方法,进行不同提取条件时,药物中多糖的含量比较.方法采用苯酚-硫酸比色法,于490nm处测定含量.结果该方法线性关系良好,平均加样回收率为100.5%,RSD＝1.23%.结论本法准确,可作为雷公藤中多糖的质量控制.
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| 关 键 词： | 雷公藤 多糖 含量测定 |
| 文章编号： | 1007-7693(2004)01-0008-03 |
Extraction and determination of polysaccharide from Tripterygium wilfordii |
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| LIANG Hui-hu,LIU Xiao-he,WANG Zhi-bao,GUO Chun-yan. Extraction and determination of polysaccharide from Tripterygium wilfordii[J]. The Chinese Journal of Modern Applied Pharmacy, 2004, 21(1): 8-10 |
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| Authors: | LIANG Hui-hu LIU Xiao-he WANG Zhi-bao GUO Chun-yan |
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| Abstract: | OBJECTIVE To establish the method for determination of polysaccharide from Tripterygium silfordii Hook.f., and compare the content of Tripterygium silfordii Hook.f. polysaccharide extracted with different methods. METHOD The content of polysaccharide was determined by phonel-sulfuric acid method, the detection wavelength was 490nm.RESULTS The dinear relationship of this method was well and the average recovery was 100.5%, RSD=1.23%.CONCLUSION This method is accurate and can be used for quality control of polysaccharide in Tripterygium silfordii Hook.f.. |
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| Keywords: | Tripterygium wilfordii Hook.f. polysaccharide determinate |
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