细胞凋亡基因及相关蛋白在早期自然流产胎盘绒毛和蜕膜组织中的表达

目的探讨自然流产和正常人流患者胎盘绒毛和蜕膜组织细胞增殖与凋亡的变化规律,及相关细胞因子在胎盘组织生长发育中的表达意义。方法应用免疫组织化学、末端脱氧核糖核酸转移酶介导的缺口末端标记法（terminal de-oxynucleotidy transferase mediated dUTP-biotin nick end labing,TUNEL）检测第40-90天自然流产和正常人流患者胎盘组织中细胞凋亡的表达状况。结果第40-90天的正常人流患者的绒毛和蜕膜组织细胞的增殖占主导地位,大部分细胞增殖活跃,细胞的增殖与凋亡始终处于一种动态平衡;而自然流产患者的绒毛和蜕膜组织中,凋亡细胞的比例明显增大,细胞增殖与凋亡的动态平衡被打破。结论妊娠妇女胎盘组织内细胞凋亡比例增高,细胞增殖与凋亡的动态平衡被打破是自然流产发生的基础。

The expresston of apoptosis gene and related proteins in the early placental chorionig villi and decidual tissues of spontaneous abortion

Objective： To investigate the role of cell proliferation and apoptosis of placental chorionic villi and decidua of the spontaneous abortion and normal human abortion , and the expression significance of involved Cytokines in the placental growth and development . Methods ： The expression of apoptosis in the placenta of spontaneous abortion and normal abortion was detected from the 40th to the 90th day by immunohistochemical methods, terminal deoxynucleotidy transferase mediated dUTP - biotin nick end labing （TUNEL）. Results： From the 40th to the 90th day , cborionic villi and decidua of the normal abortion dominated by the majority of active cell proliferation, cell proliferation and apoptosis always in a dynamic equilibrium; and the proportion of apoptotic cells of spontaneous abortion decidua and chorionic villi was increased , and cell proliferation and apoptosis of dynamic balance was broken. Conclusions ： The placenta of pregnant women with higher proportion of apoptosis, a dynamic equilibrium of cell proliferation and apoptosis is broken on the basis of the occurrence of spontaneous abortion.