土茯苓抗人巨细胞病毒的体外实验研究

目的:探讨土茯苓体外抗HCMV的效果和安全性。方法:用细胞病变法和MTT法,检测土茯苓体外抗人巨细胞病毒的最大无毒浓度、最小有效浓度和治疗指数,并与更昔洛韦、复方中药金叶败毒进行比较。结果:更昔洛韦、金叶败毒、土茯苓的最大无毒浓度分别为10μg/ml、3000μg/ml、30000μg/ml,最小有效浓度分别为10μg/ml、300μg/ml、300μg/ml,治疗指数分别为1、10、100。结论:土茯苓细胞毒性略低于金叶败毒,大大低于更昔洛韦;抗HCMV效果与金叶败毒相同,略低于更昔洛韦;治疗指数最高,有可能成为治疗HCMV活动性感染的药物。

Experimental study on the anti-cytomegalovirus effect of smilax glabra in vitro

Objective:To explore the anti-cytomegalovirus efficacy and safety of smilax glabra in vitro.Methods:Cytopathic assay and MTT method were used to detect the maximum atoxic concentration,minimum effective concentration and therapeutic index of anti-cytomegalovirus effect of smilax glabra in vitro,then the results were compared with ganciclovir and compound Jinyebaidu.Results:The maximum atoxic concentrations of ganciclovir,compound Jinyebaidu and smilax glabra were 10 μg/ml,3 000μg/ml and 30 000μg/ml,respectively;the minimum effective concentrations of ganciclovir,compound Jinyebaidu and smilax glabra were 10 μg/ml,300 μg/ml and 300 μg/ml,respectively;the therapeutic indexes of ganciclovir,compound Jinyebaidu and smilax glabra were 1,10 and 100,respectively.Conclusion:The cytotoxicity of smilax glabra is lower than that of compound Jinyebaidu,which is significantly lower than that of ganciclovir;the anti-cytomegalovirus effect of smilax glabra is similar to that of compound Jinyebaidu,which is lower than that of ganciclovir;the therapeutic index of smilax glabra is the highest,which may become to be a new drug for treatment of active infection of cytomegalovirus.