| 高效液相色谱法测定妇乐颗粒中大黄素和大黄酚含量 |
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| 引用本文： | 盛燕,汪培钧,张文婷,陈勇.高效液相色谱法测定妇乐颗粒中大黄素和大黄酚含量[J].中国药业,2010,19(8):46-47. |
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| 作者姓名： | 盛燕 汪培钧 张文婷 陈勇 |
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| 作者单位： | 1. 浙江省食品药品检验所,浙江杭州,310004
2. 杭州天目山药业股份有限公司,浙江,临安,311300 |
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| 摘 要： | 目的 建立测定妇乐颗粒中大黄素和大黄酚含量的高效液相色谱（HPLC）法。方法色谱柱为Zorbax Extend—C18柱（250mm×4．6mm，5μm），流动相为甲醇-0．1％磷酸溶液（80：20），流速为1．0mL／min，柱温为30℃，检测波长为265nm。结果大黄素的进样量线性范围为0．01054—0．21080μg，r=0．9999（H=6），平均回收率为101．69％，RSD=2．52％（n=6）；大黄酚的进样量线性范围为0．01112—0．22240μg，r=1．0000（n=6），平均回收率为96．73％，RSD=1．74％（n=6）。结论该方法结果准确，重现性好，可作为妇乐颗粒质量控制的定量方法之一。
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| 关 键 词： | 妇乐颗粒 高效液相色谱法 大黄素 大黄酚 |
Determination of Emodin and Chrysophanic Acid in Fule Granules by HPLC |
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| Sheng Yan,Wang Peijun,Zhang Wenting,Chen Yong.Determination of Emodin and Chrysophanic Acid in Fule Granules by HPLC[J].China Pharmaceuticals,2010,19(8):46-47. |
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| Authors: | Sheng Yan Wang Peijun Zhang Wenting Chen Yong |
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| Institution: | 1. Zhejiang Institute for Food and Drug Control, Hangzhou, Zhejiang, China 310004; 2. Hangzhou Tianmushan Pharmaceutical Co., Ltd., Lin＇an, Zhejiang, China 311300) |
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| Abstract: | Objective To establish a HPLC method for the determination of emodin and chrysophanic acid in Fule Granules. Methods The samples were analyzed on the Zorbax Extend C18 column （250 mm ×4.6 mm, 5 μm）.The mobile phase was the mixture of MeOH-0. 1% H3PO4（80 ： 20）, the flow rate was 1.0 mL/min, the column temperature was 30 ℃, the detection wavelength was 265 nm. Results The calibration curve was linear in the range of 0. 010 54 μg - 0. 210 80 μg, r = 0. 999 9（ n = 6） for emodin, and 0.011 12 μg - 0. 222 40 μg, r = 1. 000 0（ n = 6） for chrysophanic acid. The recovery rate was 101.69%, RSD = 2.52% for emodin, and 96. 73%, RSD = 1.74% （ n = 6） for chrysophanic acid. Conclusion The method is sensitive and its results are accurate with good reproducibility. It can be used for the quality control of Fule Granules. |
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| Keywords: | Fule Granules HPLC emodin chrysophanie acid |
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