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| 牙龈成纤维细胞的肝细胞生长因子分泌方式的实验观察 | |
| 引用本文： | 武志强,凌丽,Thomas DW. 牙龈成纤维细胞的肝细胞生长因子分泌方式的实验观察[J]. 牙体牙髓牙周病学杂志, 2000, 10(5): 262-264 |
| 作者姓名： | 武志强 凌丽 Thomas DW |
| 作者单位： | 1. 中国医科大学第二临床学院口腔科 2. 市口腔医院，辽宁沈阳110002 3. 英国威尔士大学口腔医学院，Cardiff，CF4 4XY |
| 摘 要： | 目的：观察牙龈成纤维细胞的肝细胞生长因子（ＨＧＦ／ＳＦ）的分泌方式。方法：培养牙龈成纤维细胞；制作该细胞胶原网架（ＦＰＣＬｓ），将ＦＰＣＬｓ分组，从ＦＰＣＬ形成后的０～７ｄ，每天从３个不同角度测量ＦＰＣＬ的直径缩小值。结果：不含血清培养液（ＳＦＭ）组与ＳＦＭ＋ｒＨＧＦ组ＦＰＣＬ的收缩变化都很小，两组间无差异（Ｐ〉０．０５）；而含血清培养液（ＳＣＭ）组的收缩变化非常大，与其它两组间具有极显著差异（Ｐ |
| 关 键 词： | 肝纤维生长因子 牙龈成纤维细胞 细胞培养 |
| 文章编号： | 1005-2593(2000)05-0262-03 |
| 修稿时间： | 1999-11-02 |
An investigation to the secretion mode of Hepatocyte Growth Factor/Scatter Factor by gingival fibroblasts in vitro |
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| WU Zhi-qiang,LING Li,Thomas DW. An investigation to the secretion mode of Hepatocyte Growth Factor/Scatter Factor by gingival fibroblasts in vitro[J]. Chinese Journal of Conservative Dentistry, 2000, 10(5): 262-264 | |
| Authors: | WU Zhi-qiang LING Li Thomas DW |
| Abstract: | AIM:To investigate the secretion mode of hepatocyte growth factor/scatter factor(HGF/SF) by gingival fibroblasts in vitro . METHODS: Gingival fibroblasts were cultured, and the fibroblast populated collagen lattices (FPCLs) were farbricatad and divided into 3 groups. The diameter of the FPCL was measured from day 0 to day 7, and the degree of cell-mediated reorganization was estimated with three separate lattice diameter measurements. RESULTS: No differences in the initial rate of lattice reorganization were observed between fibroblasts in serum-free median with and without 20 (or 40)ng/ml rHGF groups ( P >0.05) ;culture in the presence of serum significantly stimulated lattice reorganization( P <0.01). CONCLUSION: rHGF does not modulate fibroblast-mediated extracellular matrix reorganization. This study does not provide the evidence for the autocrine of HGF/SF by gingival fibroblasts. [ |
| Keywords: | hepatocyte growth factor/scatter factor fibroblast populated collagen lattice gingival fibroblast cell culture |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |