Évolution des souches de Rotavirus du groupe A en circulation en Tunisie sur une période de trois ans (2005–2007) |
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| Authors: | A. Chouikha M. Ben Hadj Fredj I. Fodha I. Mathlouthi M. Ardhaoui N. Teleb I. Brini F. Messaadi M. Mastouri T. Sfar M. Hachicha T. Kammoun A. Bouaaziz F. Amri A. Harbi M. Zribi S. Bousnina A. Khemakhem N. Boujaafar A. Trabelsi A.D. Steele |
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| Affiliation: | aLaboratoire MDT-01, faculté de pharmacie, Monastir, Tunisie;bUR06/SP20, laboratoire de microbiologie, CHU Sahloul, 4059 Sousse, Tunisie;cService de pédiatrie, hôpital d’Enfants, Tunis, Tunisie;dVaccine Preventable Diseases and Immunization, WHO/EMRO, Le Caire, Égypte;eLaboratorire d’Hygiène, CHU Hedi Chaker, Sfax, Tunisie;fLaboratoire de microbiologie, CHU Fattouma Bourguiba, Monastir, Tunisie;gService de pédiatrie, CHU Tahar Sfar, Mahdia, Tunisie;hService de pédiatrie, CHU Hedi Chaker, Sfax, Tunisie;iService de pédiatrie, CHU Tletli, Nabeul, Tunisie;jService de pédiatrie, CHU Ibn Al Jazzar, Kairouan, Tunisie;kService de pédiatrie, CHU Sahloul, Sousse, Tunisie;lGeneral Direction of Health, Ministry of Public Health, Tunis, Tunisie;mVaccines and Immunization, PATH, Seattle, États-Unis |
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| Abstract: | BackgroundRotaviruses are the most frequent agents associated with diarrhoea in children worldwide. Analysis of mobility of the 11 segments of genomic RNA by polyacrylamide gel electrophoresis (PAGE) yields a pattern which is characteristic for a particular rotavirus isolate. The group A rotaviruses can be further characterized by analysis of VP7 and VP4 genes specificities, responsible for rotavirus classification into G and P genotypes, respectively. The aim of the present study was to determine the evolution of group A Rotavirus strains circulating in Tunisia over a 3-year period (2005–2007).Material and methodsA total of 1503 stool samples collected from children less than five years old, consulting or hospitalised in Tunisia for diarrhoea between 2005 and 2007, were screened for the presence of group A Rotaviruses. Rotavirus-positive specimens were further analyzed by PAGE and G/P-genotyped by multiplex semi-nested RT-PCR.ResultsRotaviruses were detected in 323 stool samples over 1503 (21 %). Long electropherotypes predominated in Tunisia during the whole period of study (N = 158 vs N = 82 short electropherotypes). VP7 genotyping showed the cocirculation of five different genotypes: G1, G2, G3, G4 and G9. VP4 typing detected four different P-genotypes: P[8], P[4], P[6] and P[11]. Rotavirus strains with G3P[8] specificity were predominating in Tunisia in 2005 and 2006, replaced by G2P[4] strains in 2007. |
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| Keywords: | Mots clé s: Rotavirus Gastroenté rite É lectrophoré type Gé notypage VP7 VP4 |
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