单纯多发性肌炎肌肉组织中趋化因子的表达

目的 探讨趋化因子单核细胞趋化蛋白－1（MCP－1）、巨噬细胞炎症蛋白－1α（MIP－1α）和调节活化正常T细胞表达分泌的因子（RANTES）在单纯多发性肌炎（SPM）受累肌肉组织中的表达状况，并着重分析它们与SPM病期、病情的关系。方法 采用免疫组织化学LAB法，观察这些趋化因子蛋白在SPM受累肌肉组织中的表达状况，结合图像分析处理系统定量其表达水平。结果 这些趋化因子蛋白在SPM中均有表达；MCP－1在慢性组和病情较重组表达显著增加（均P＜0．01），MIP－1α在病情较轻的慢性患者中表达显著增加（P＜0．05），RANTES在不同病期组的表达无显著性差异（均P＞0．05）。结论 MCP－1可能不但在SPM病程的慢性进展中起较大作用，而且与病情严重程度有关，可作为病情进展或恶化的一个指标；MIP－1α则可能参与了疾病的持续发展；RANTES在SPM发病过程中可能只是机体对炎性损伤的一种普遍性反应。

The expression of chemokine factor in the muscular tissue of simple polymyositis

Objective To detect the protein expression of chemotactic monocyte chemotactic protein 1 (MCP 1), macrophage inflammatory protein 1 alpha (MIP 1alpha) and regulated on activation normal T cell expression secretion (RANTES) in the affected muscle of simple polymyositis (SPM), and analysed the relationship between the amount of these proteins and the stage, severity of SPM.Methods Immuohistochemical LAB technique was used to detect the expression of these chemotactic factor protein in SPM affected muscle specimens.The expression levels of these chemotactic factors were determined by Image Analyzer system.Results The expressions of these chemotactic factors protein in SPM were showed in SPM. The expression levels of MCP 1 increased remarkably in the chronic SPM group and the severer one (P<0 01). The expression of MIP 1alpha increased remarkably in the slight patients of chronic group( P <0 05), but the expression of RANTES showed no significant difference between the groups divided by stage or severity(P>0 05).Conclusion MCP 1 not only could play an important role in the chronic SPM,but also related to the severity of the disease,it might be as an index on the development and deterioration of the di sease;MIP 1alpha might contribute to the continuous development of the disease. RANTES might embody its role as a ubiquitous response to inflammation during the onset of SPM.