| 柱前衍生HPLC法测定地龙中氨基酸的含量 |
| |
| 引用本文： | 裴福成,李长新,任桂萍. 柱前衍生HPLC法测定地龙中氨基酸的含量[J]. 中医药学报, 2007, 35(3): 26-27 |
| |
| 作者姓名： | 裴福成 李长新 任桂萍 |
| |
| 作者单位： | 1. 哈药集团中药二厂,黑龙江,哈尔滨,150078
2. 东北农业大学,黑龙江,哈尔滨,150030 |
| |
| 摘 要： | 目的：分析地龙中氨基酸的含量及其特征性。方法：采用邻苯二甲醛（OPA）、9-芴甲基氯甲酸酯（FMOC）联合柱前衍生HPLC测定地龙中的17种氨基酸,峰面积外标法定量。结果：各氨基酸在26min内可得到较好分离,氨基酸浓度与峰面积呈良好的线性关系,r值均在0.995以上。结论：所建立的方法分离效果好、灵敏、准确、简便,适宜地龙药材中氨基酸的测定。
|
| 关 键 词： | 地龙 氨基酸 高效液相色谱 柱前衍生 |
| 文章编号： | 1002-2392（2007）03-0026-02 |
| 修稿时间： | 2006-12-052007-03-06 |
Determination of amino acids in Pheretima aspergillum by pre - column derivatization of HPLC |
| |
| PEI Fu-cheng,LI Chang-xin,REN Gui-ping. Determination of amino acids in Pheretima aspergillum by pre - column derivatization of HPLC[J]. Acta Chinese Medicine and Pharmacology, 2007, 35(3): 26-27 |
| |
| Authors: | PEI Fu-cheng LI Chang-xin REN Gui-ping |
| |
| Abstract: | Objective:To determine the contents of the amino acids and study the characters of Pheretima aspergillum(E.Perrier). Methods:The samples of Pheretima aspergillum(E.Perrier) were derived with o-phthaldialdehyde (OPA) and 9-fluorenylmethyl chloroformate (FMOC) in pre-column , and separated by HPLC. Seventeen kinds of amino acids were determined with the ration of peak area by external standard calibration.Results:All kinds of amino acids can be well separated in 26 min,the concentration of amino acids and peak areas achieved a nice linear relations ( r is higher than 0.995).Conclusion:The method is sensitive,accurate,simple,and reliable for the determination of amino acids in Pheretima aspergillum(E.Perrier). |
| |
| Keywords: | Pheretima aspergillum(E.Perrier) amino acid HPLC pre-column derivatization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
