siRNA沉默Snail基因对胃癌SGC7901细胞株增殖及侵袭能力的影响

目的探讨转录因子Snail在胃癌SGC7901细胞株增殖及侵袭转移过程中的作用,为胃癌的基因治疗提供有效靶点。方法化学合成针对Snail的靶向siRNA,同时以转染阴性对照siRNA、转染脂质体转染试剂和空白细胞作为对照。RT-PCR检测转染效率,MTT检测Snail siRNA对SGC7901细胞增殖能力的影响,Boyden chamber检测Snail siRNA对SGC7901细胞侵袭能力的影响。结果与各对照组相比,转染SnailsiRNA组SGC7901细胞Snail mRNA的表达下降,且随转染时间延长,Snail mRNA的表达下降明显(P<0.05)。MTT结果显示转染Snail siRNA组SGC7901细胞在转染后48、72 h较对照组增殖能力明显下降(P<0.05);Boyden chamber结果显示转染Snail siRNA组SGC7901细胞在转染后48、72 h较对照组穿膜细胞数明显下降(P<0.05);转染Snail siRNA组各时间点之间增殖能力和侵袭转移能力的差异也有统计学意义(P<0.05)。结论转录因子Snail在胃癌的发生、发展及侵袭转移中发挥重要作用,可作为胃癌基因治疗的有效靶点。

The Effect of Silencing Snail by siRNA on Generation and Invasion Capability of Gastric Cancer SGC7901 Cell Line

Objective To explore the effect of silencing Snail by siRNA on the generation and invasion capability of cell line SGC7901 in vitro,and to supply a specific target in gene therapy of gastric cancer.Methods Synthesized siRNA targeting Snail were transfected into SGC7901 cells,at the same time,SGC7901 cells transfected with negative siRNA,SGC7901 cells transfected with Lipofectamine 2000 and vacant SGC7901 cells were used as controls.RT-PCR was used to observe the transfection efficiency.MTT was used to evaluate the generation capability and Boyden chamber was used to evaluate the invasion capability of SGC7901 cells in vitro.Results Compared to all the control groups,SGC7901 cells transfected with Snail siRNA can suppress the expression of Snail mRNA in vitro,and along with the interfering time extending,Snail mRNA decreased gradually(P<0.05).The results of MTT showed that the generation ability of SGC7901 cells decreased greatly after transfected Snail siRNA for 48 and 72 h compared to those in all the control groups(P<0.05);the results of Boyden chamber also showed that the invasion ability of SGC7901 cells decreased greatly after transfected Snail siRNA for 48 and 72 h compared to those in all the control groups(P<0.05);and there were significant variance among 24,48 and 72 h(P<0.05).Conclusion Snail play an important role in the generation,development and invasion of gastric cancer,and may be a potentially valuable therapeutic target of gastric cancer.