NS-398对人胃癌细胞系SGC-7901增殖、凋亡和COX-2表达的影响

目的 探讨环氧合酶-2(COX-2)抑制剂NS-398对人胃癌细胞系SGC-7901增殖、凋亡及COX-2表达的影响,并进一步探讨其作用的可能机制.方法 采用四甲基偶氮唑蓝(MTT)法检测NS-398对SGC -7901细胞的杀伤抑制作用；免疫细胞化学法检测SGC-7901细胞内COX-2的蛋白表达情况；ELISA法检测NS-398作用于SGC-7901细胞后PGE2释放水平；流式细胞仪检测SGC-7901细胞的凋亡情况.结果 NS-398对胃癌SGC-7901细胞具有较强的抑制作用,且这种抑制作用随浓度和时间的增加而增强,呈剂量-时间双效应关系(P＜0.05)；不同浓度NS-398作用下的SGC-7901细胞中,COX-2的表达明显减弱,且呈剂量梯度下降(P＜ 0.05)；NS-398可抑制PGE2释放,并且这种抑制作用呈剂量效应关系,与对照组相比差异有统计学意义(P＜ 0.05)；NS-398作用于SGC-7901细胞48 h后,细胞凋亡率升高,且呈剂量效应(P＜0.05).结论 NS-398通过COX-2依赖途径抑制SGC-7901细胞增殖并促进其凋亡.

The Effect of NS-398 on Proliferation, Apoptosis and Expressions of COX-2 of Human Gastric Cancer SGC-7901 Cells

Objective To investigate the effect of cyclooxygenase-2(COX-2) inhibitor NS-398 on the proliferation,apoptosis and cox-2 expressions of human gastric cancer SGC-7901 cells,and to explore its possible mechanism. Methods Methyl thiazol tetrazolium(MTT) method was used to detect the antiproliferative ratio of NS-398 on SGC-7901 cells;immunocytochemical method was used to detect the COX-2 protein expressions;ELISA method was used to detect the release levels of prostaglandin E2(PGE2) of human gastric cancer SGC-7901 cells given with NS-398;flow cytometry was used to analyze the gastric cancer cell apoptosis. Results NS-398 inhibited the proliferation of SGC-7901 cells in a time-and dose-dependent manner(P<0.05).The expressions of COX-2 significantly decreased after the administration of NS-398,a dose-dependent manner was observed(P<0.05).NS-398 significantly inhibited the release of PGE2 in SGC-7901 cells in a dose-dependent manner(P<0.05).The apoptosis of SGC-7901 cells increased after the administration of NS-398 for 48 hours,and the apoptosis increased more and more with the dose of NS-398(P<0.05). Conclusion NS398 can inhibit the proliferation of human gastric cancer SGC-7901 cells and induce the apoptosis,which may contribute to the COX-2 dependent pathway.