鱼藤素对肝癌细胞株SMMC-7721增殖抑制和凋亡诱导作用的研究

目的 研究鱼藤素对肝癌细胞株SMMC-7721增殖和凋亡的影响及其作用机制.方法 MTT法检测鱼藤素对SMMC-7721细胞增殖的抑制作用;DNA琼脂糖凝胶电泳观测细胞凋亡情况;采用Western blotting法检测相关蛋白表达水平的变化,探讨其作用机制.结果 鱼藤素呈剂量和时间依赖性抑制SMMC-7721细胞增殖,诱导细胞凋亡致DNA ladder形成,出现相应的形态学改变;鱼藤素可明显降低Akt的磷酸化水平.结论 鱼藤素可通过下调Akt的活性,抑制SMMC-7721的增殖并诱导细胞凋亡,或可成为一种新的抗肝癌药物.

Study of Proliferation Inhibition and Apoptosis Induction of Deguelin in Human Hepatocellular Cancer Cell Line SMMC-7721

Objective To investigate the effects of deguelin on proliferation and apoptosis of human hepatocellular cancer cell line SMMC-7721.Methods The proliferation inhibition of deguelin in SMMC-7721 cells was determined by the MTT assay.Apoptosis was determined by Agarose gel DNA electrophoresis.The expression of related proteins was determined by Western blotting.Results Deguelin inhibited SMMC-7721 cells growth in does-and time-dependent manner and caused apoptotic death of SMMC-7721 cells.These effects were characterized by significantly decreased level of phosphorylated Akt.Conclusion Deguelin inhibited proliferation and induced apoptosis in SMMC-7721 cells via downregulation of activity of Akt,and it might be a potential agent against hepatocellular cancer.