椒梅连理汤抗慢性溃疡性结肠炎的实验研究

目的 观察椒梅连理汤抗实验性溃疡性结肠炎的作用,探讨其治疗作用可能的免疫学机制.方法 将43只SD大鼠随机分为4组:正常组10只,模型组、柳氮磺胺吡啶组(SASP组)、椒梅连理汤组(中药组)各11只.采用三硝基苯磺酸法制备大鼠溃疡性结肠炎模型,分别予以相应药物1 ml/100 g灌胃,每天1次,连续4周.检测大鼠血清及结肠黏膜中白介素1β(IL-1β)、白介素4(IL-4)、白介素6(IL-6)、肿瘤坏死因子α(TNF-α)的含量.结果 模型组大鼠肠黏膜出现充血、水肿、增生和溃疡,SASP组和中药组较模型组明显好转.与正常组比较,模型组大鼠IL-1β、IL-6、TNF-α明显升高(P<0.05),IL-4明显下降(P<0.05),SASP组、中药组和模型组比较,IL-1β、IL-6、TNF-α降低(P<0.05),IL-4升高(P<0.05).SASP组和中药组之间比较差异无统计学意义(P＞0.05).结论 椒梅连理汤能有效对抗实验性溃疡性结肠炎,与SASP相当,通过调节IL-1β、IL-6、TNF-α、IL-4含量可能是其治疗机制之一.

Experiments on Jiaomei Lianli Decoction in the treatment of chronic ulcerative colitis

Objective To observe the anti-ulcerative colitis effects of Jiaomei Lianli Decoction, and to discuss the possible immunological mechanisms of the treatment effect. Methods 43 SD rats were randomly divided into four groups, normal group （ n = 10 ）, model group （ n = 11 ）, salicylazosulfapyridine group （ SASP group） （ n = 11 ）, Jiaomei Lianli Decoction group （ TCM group） （ n = 11 ）. The rat ulcerative colitis model was established by using 2.4.6-trinitrobenzene sulfonic acid （TNBS）. Corresponding drugs were respectively administered by gastric infusion to the groups for 1 ml/100 g one day for four weeks. Content of IL-1β, IL-4, IL-6, TNF alpha in serum and colonic mucosa were detected. Results There were congestion, edema, proliferation and ulcers in colonic mucosa of model rat, while the conditionS of SASP group and TCM group were improved obviously. Compared with normal group, contents of IL-1β, IL-6, TNF alpha of model group were increased significantly （P〈0.05）, content of IL-4 declined obviously （P 〈 0.05）. Compared with model group, contents of IL-1β, IL-6, TNF alpha of SASP group and TCM group declined significantly （ P〈0.05 ）, content of IL-4 increased obviously （ P〈0.05 ）. There were no significant differences between SASP group and TCM group （P 〉 0.05 ）. Conclusion Jiaomei Lianli Decoction demonstrated effective anti-ulcerative colitis by adjusting the content of IL-1β, IL-6, TNF alpha and IL-4, which may be one of the treatment mechanisms, and the effects were similar to SASP.