黄芪甲苷和三七总皂苷中主要有效成分抗PC12细胞氧化损伤的配伍研究

目的 探索黄芪甲苷和三七总皂苷中主要有效成分人参皂苷Rg1、人参皂苷Rb1、三七皂苷R1抗CoCl2 诱导的PC12细胞氧化损伤的有效配伍剂量.方法 采用CoCl2诱导PC12细胞氧化损伤模型,以细胞乳酸脱氢酶(LDH)漏出率为指标,研究4种有效成分对PC12细胞氧化损伤的有效机制浓度.在此基础上按Us*(85)均匀设计实验,确定4种有效成分的有效配伍剂量.结果 4种有效成分都能抑制CoCl2诱导的PC12细胞LDH的漏出,与损伤组相比差异有统计学意义(P＜0.05).且随着药物浓度的增加,对LDH漏出率的抑制作用增强.黄芪甲苷和人参皂苷Rb1在50 μmol/L,人参皂苷Rg1和三七皂苷R1在100 mol/L浓度时LDH漏出抑制率约为80％.U8*(85)均匀设计实验多元逐步回归分析得:4种有效成分的8个不同浓度配伍都能抑制CoCl2诱导的PC12细胞LDH的释放,与损伤组相比差异有统计学意义(P＜0.05).人参皂苷Rg150 μmol/L、黄芪甲苷0.781 25 μmogL、人参皂苷Rb1和三七皂苷R1各1.562 5μ.mol/L配伍时抗PC12细胞氧化损伤的效应最强.结论 人参皂苷Rg1 50 μmol/L、黄芪甲苷0.78125 μmol/L、人参皂苷Rb1和三七皂苷R1各1.562 5μmol/L配伍组方为抗PC12细胞氧化损伤的有效配伍剂量.

Compatibility study of main effective components in PNS and AST on oxidative damage of PC12

Objective To investigate the effective compatibility dose of GinsenosideRg1,GinsenosideRb1,AstragalosideⅣ and NotoginsenosideR1 against CoCl2-induced oxidative injury on PC12 cells.Methods The Lactate dehydrogenase(LDH) leakage ratio was calculated after LDH levels in PC12 cells induced by CoCl2 and cell culture medium were determined.The effective concentration of four active ingredients of anti-oxidative damage in PC12 cells were determined by LDH leakage rates and then the best dose compatibility of these four active ingredients was identified according to the uniform design of U8*(85).Results Compared with model group,all the eight different concentrations of the four active ingredients could effectively inhibit the release of LDH(P<0.05).Furthermore,the inhibiting rates of the LDH leakage were increased with the increasing doses.The inhibition of LDH leakage rate in PC12 cells was 80% treated with 50 μmol/L AstragalosideⅣ and GinsenosideRb1,100 μmol/L GinsenosideRg1 and NotoginsenosideR1.It showed that eight different concentrations of compatibility of four active ingredients could effectively inhibit the release of LDH and there were statistical significance between medicine groups and model group(P<0.05) analyzed by multiple regression analysis.There was best effect of anti-oxidative damage of PC12 cells induced by CoCl2 when treated with 50 μmol/L GinsenosideRg1,0.78125 μmol/L Astragaloside Ⅳ,1.5625 μmol/L GinsenosideRb1 and NotoginsenosideR1.Conclusion The effective components compatibility doses of 50 μmol/L GinsenosideRg1,0.78125 μmol/L Astragaloside Ⅳ,1.5625 μmol/L GinsenosideRb1 and NotoginsenosideR1 are the best effective dose for anti-oxidative damage of PC12 cells induced by CoCl2.