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| 神经节苷脂诱导胶质瘤细胞自噬性死亡 | |
| 引用本文： | 马明明,王雪晶,丁雪冰,张钱林,贺爽,张杰文.神经节苷脂诱导胶质瘤细胞自噬性死亡[J].肿瘤基础与临床,2012,25(4):281-283. |
| 作者姓名： | 马明明 王雪晶 丁雪冰 张钱林 贺爽 张杰文 |
| 作者单位： | 1. 河南省人民医院神经内科,河南,郑州,450003 2. 郑州大学第一附属医院神经内科,河南,郑州,450052 3. 河南省人民医院肾脏内科,河南,郑州,450003 |
| 摘 要： | 目的 探讨神经节苷脂(GM1)对恶性脑胶质瘤细胞U251自噬性死亡的影响.方法 GM1作用U251细胞后,应用MTT法检测细胞的存活率,流式法检测细胞凋亡的改变,荧光显微镜下观察MDC染色后细胞质内酸性自噬泡的形成情况及外源性LC3-Ⅱ的表达;Western blotting法检测细胞自噬相关蛋白LC3-Ⅱ/LC3-Ⅰ的比值、Lamp-2a、Beclin-1的表达.结果 与对照组相比,GM1作用48 h后,U251细胞存活率明显降低,且具有剂量依赖性,差异有统计学意义(P<0.05);与对照组相比,GM1作用48 h后,U251细胞凋亡率明显升高,且具有剂量依赖性,差异有统计学意义(P<0.05);GM1作用后细胞内LC3-Ⅱ/LC3-Ⅰ的比值、Lamp-2a及Beclin-1的表达水平明显上调,差异有统计学意义(P<0.05).结论 GM1可以诱导恶性脑胶质瘤细胞U251自噬性死亡,为恶性脑胶质瘤的治疗提供新的方向. |
| 关 键 词： | 自噬性死亡 神经节苷脂 U251细胞 |
Autophagic Cell Death Induced By Ganglioside |
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| Ma Mingming , Wang Xuejing , Ding Xuebing , Zhang Qianlin , He Shuang , Zhang Jiewen.Autophagic Cell Death Induced By Ganglioside[J].journal of basic and clinical oncology,2012,25(4):281-283. | |
| Authors: | Ma Mingming Wang Xuejing Ding Xuebing Zhang Qianlin He Shuang Zhang Jiewen |
| Institution: | 1(1.Department of Neurology,Henan Provincial People’s Hospital,Zhengzhou 450003,China; 2.Department of Neurology,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China; 3.Department of Nephrology,Henan Provincial People’s Hospital,Zhengzhou 450003,China) |
| Abstract: | Objective To investigate the effect of monosialotetrahexosy 1 ganglioside(GM1) on autophagic cell death in malignant glioma U251 cells treated with GM1.Methods The viability of cells was measured by MTT assay.The cell apoptosis was assayed by flow cytometry.Autophagic features were determined by fluorescence microscope.The autophagy-related protein expressions of LC3-Ⅱ/LC3-Ⅰ,Lamp-2a and Beclin-1 were assessed by Western blotting.Results After treatment of GM1 for 48 hours,the growth of U251 cells were significantly inhibited in dose-dependent manner(P<0.05);and GM1 increased autophagic cell death also in dose-dependent manner(P<0.05).Compared with the control group,the levels of LC3-Ⅱ/LC3-Ⅰ,Lamp-2a and Beclin-1 were significantly increased in GM1 treatment groups(P<0.05).Conclusion GM1 enhances autophagic cell death of malignant glioma U251 cells,which provides a novel strategy to treat drug-resistant malignant glioma. |
| Keywords: | autophagic cell death monosialotetrahexosy 1 ganglioside U251 cells |
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