| 光动力疗法对人膀胱癌细胞胞内游离钙的影响 |
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| 引用本文： | 刘树硕,杨永华,裘顺安,王慎鸿,王泽时.光动力疗法对人膀胱癌细胞胞内游离钙的影响[J].中国中西医结合外科杂志,2004,10(1):32-33. |
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| 作者姓名： | 刘树硕 杨永华 裘顺安 王慎鸿 王泽时 |
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| 作者单位： | 浙江中医学院附属医院泌尿外科,杭州,310006 |
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| 摘 要： | 目的:探讨光动力学作用后的膀胱癌细胞(T-24和SCaBER)胞内钙离子浓度的变化.方法:采用MTT比色分析法判断光动力学对体外人膀胱癌T-24和SCaBER细胞的杀伤效应.同时应用激光共聚焦显微镜和Fluo-3/AM探针技术测定光动力学作用后细胞内钙离子的浓度.结果:T-24和SCaBER细胞内钙离子有明显升高,与对照组比较差异有显著意义(P＜0.001).结论:光动力学作用后细胞内钙离子超载可能在细胞死亡中发挥重要作用.
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| 关 键 词： | 膀胱肿瘤 叶绿素衍生物 光动力疗法 钙超载 激光共聚焦显微镜 |
| 文章编号： | 1007-6948(2004)01-0032-02 |
| 修稿时间： | 2003年7月19日 |
Effect of Photodynamic Therapy on Free Ca2+ in Human Bladder Cancer Cells |
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| Liu Shushuo,Yang Ronghua,Qiu Shunan,et al.Effect of Photodynamic Therapy on Free Ca2+ in Human Bladder Cancer Cells[J].Chinese Journal of Surgery of Integrated Traditional and Western Medicine,2004,10(1):32-33. |
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| Authors: | Liu Shushuo Yang Ronghua Qiu Shunan |
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| Abstract: | ObjectiveTo investigate the changes in Ca 2 ]i in bladder cancer cells (T-24 and SCaBER cell)after photodynamic therapy(PDT )MethodsThe photodynamic cytotoxity of human bladder cancer cells(T- 24 and SCaBER) was studied by MTT assay.The change in Ca 2 ]i in bladder cancer cells following PDT was detected by means of a confocal laser scanning m icroscope using the Ca 2 indicator Fluo-3/AM staining.ResultsIntracellular calcium concentration in T-24 cells and SCaBER cells increas ed markedly as compared with that in control cells( P <0 001).Con clusionIntracellsular calcium overload may play an important role in PDT induced cell death response processes. |
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| Keywords: | bladder cancer chloropyll derivative photodynamic t herapy calcium overload confocal laser scanning microscope |
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