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| Experimental Study of the Effect of the Bax Gene on Human Hepatocellular Carcinoma and Therapy via Hepatic Artery Delivery | |
| 基金项目： | This study was supported by the Scientific Research Grant from the Medical Institution of Jilin Province. |
| 摘 要： | |
| 关 键 词： | 医学实验 人类肝细胞癌 经肝动脉手术 治疗方法 |
| 收稿时间： | 2005-04-10 |
| 修稿时间： | 2005-05-12 |
Experimental study of the effect of the Bax gene on human hepatocellular carcinoma and therapy via hepatic artery delivery |
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| Zhi Guo,Wenge Xing,Haishan Yang,Lin Wang,Yunpeng Jiang,Bingyu Huang,Gang Nu,YAN Lu. Experimental study of the effect of the Bax gene on human hepatocellular carcinoma and therapy via hepatic artery delivery[J]. Chinese Journal of Clinical Oncology, 2005, 2(4): 731-736. DOI: 10.1007/BF02819539 | |
| Authors: | Zhi Guo Wenge Xing Haishan Yang Lin Wang Yunpeng Jiang Bingyu Huang Gang Nu YAN Lu |
| Affiliation: | 1. Department of Interventional Therapy,Cancer Institute & Hospital of Tianjin Medical University, Tianjin, 300060,China 2. Department of Radiology, No.3 Hospital of Jilin University, Jilin 130031,China 3. Section of Pathology, School of Basic Medical Sciences, Jilin University,Jilin 130021, China 4. Section of Genetics, No.2 Hospital of Jilin University, Jilin 130041, China |
| Abstract: | OBJECTIVE To investigate apoptosis induced by Bax in hepatocellular carcinoma cells and to examine the results of 2 different routes for in vivo gene delivery.METHODS The anti-hepatocellular carcinoma activity of the Bax gene transferred to the human hepatocellular carcinoma QGY7703 cell line was examined. In addition the Bax gene was transferred in vivo in mice via the caudal vein or hepatic artery to investigate the differences in target organ and non-target organ transfection.RESULTS 1)The Bax gene mediated by a binary adenoviral vector system induced apoptosis in the human hepatic carcinoma QFY7703 cell line. The cell apoptotic rate in the experimental group (Bax) was 50.2± 6.9% but only 32.1 ± 9.7% in the Ad/CMV-p53 group, showing that the Bax-apoptotic rate was significantly higher than the control group. 2) LacZ expression was higher in the target organ (liver) when given through the hepatic artery than through the tail vein. In contrast, LacZ expression in the nontarget organs was higher if given through the tail vein compared to the hepatic artery.CONCLUSION Superselective hepatic artery delivery with Bax gene therapy is safe, specific, effective and has low toxicity. This study provided the basis for Bex-gene therapy via the hepatic artery in vivo. |
| Keywords: | Bax hepatocellular carcinoma hepatic artery |
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