| 启动子DNA甲基化抑制白血病患者WT1基因的表达 |
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| 引用本文： | 丁超,李惠民,朱宝生,何迪,李斌.启动子DNA甲基化抑制白血病患者WT1基因的表达[J].重庆医学,2008,37(22):2561-2563. |
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| 作者姓名： | 丁超 李惠民 朱宝生 何迪 李斌 |
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| 作者单位： | 昆明医学院附属第四医院血液科,650021;云南省人民医院遗传中心,昆明,650031 |
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| 摘 要： | 目的 研究白血病患者WT1启动子区域DNA甲基化与WT1表达水平之间的关系及临床意义.方法 利用RT-PCR和甲基化特异性PCR(MSP)检测64例白血病患者、56例对照者骨髓内WT1mRNA水平及DNA甲基化状态.结果 骨髓中WT1表达率与WT1启动子DNA甲基化程度呈负相关性,在急性白血病患者(初诊、复发及未缓解者)和慢粒进入加速期和急变期患者骨髓中WT1启动子DNA甲基化率低于健康者和非白血病患者.结论 WT1启动子区域DNA甲基化抑制WT1mRNA表达.激发WT1启动子区甲基化从而抑制WT1表达,与急性白血病发生转化有关,可以作为治疗和预防白血痛发生的一个研究方向和方法.
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| 关 键 词： | 急性白血病 WT1 MSP DNA甲基化 |
The inhibitory effect of promoter DNA methylation on WT1 gene expression in patients with leukemia |
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| DING Chao,LI Hui-min,ZHU Bao-sheng,et al..The inhibitory effect of promoter DNA methylation on WT1 gene expression in patients with leukemia[J].Chongqing Medical Journal,2008,37(22):2561-2563. |
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| Authors: | DING Chao LI Hui-min ZHU Bao-sheng |
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| Institution: | DING Chao1,LI Hui-min1,ZHU Bao-sheng2,et al.(1.Department of Hematology,the Forth Affiliated Hospital of Kunming Medical College,Kunming 650021,China,2.The Hereditary Center of Yunnan Provincial People\'s Hospital,Kunming 650031,China) |
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| Abstract: | Objective To explore the correlation between DNA methylation patterns of WT1 gene promoter region and WT1 gene expression level as well as its clinical significance in patients with acute leukemia.Methods RT-PCR and methylation specific polymerase chain reaction(MSP)were applied to detecting WT1 mRNA level and DNA methylation status in 64 cases of leukemia and 56 healthy controls.Results There was negative correlation between DNA methylation status in WT1 promoter region and the level of WT1 mRNA in bone ma... |
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| Keywords: | WT1 MSP |
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