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新疆维吾尔族妇女宫颈癌组织HPV16型E6基因变异分析
引用本文:朱开春,玛依努尔·尼娅孜,刘芳,代建霞,阿丽娅·克里木,张晓玲,刘熔. 新疆维吾尔族妇女宫颈癌组织HPV16型E6基因变异分析[J]. 临床肿瘤学杂志, 2008, 13(3): 209-212
作者姓名:朱开春  玛依努尔·尼娅孜  刘芳  代建霞  阿丽娅·克里木  张晓玲  刘熔
作者单位:1. 新疆维吾尔自治区人民医院临床医学研究中心,乌鲁木齐,830001
2. 新疆维吾尔自治区人民医院妇产科,830001
摘    要:目的:新疆维吾尔族妇女宫颈癌的发生主要与人乳头状瘤病毒16(HPV16)感染相关,特定的HPV16 E6突变株具有更高的致癌危险性.本研究通过检测维吾尔族妇女宫颈癌组织中HPV16 E6基因突变情况,探讨其与维吾尔族妇女宫颈癌高发的关系.方法:从140例维吾尔族妇女宫颈癌石蜡包埋组织中提取DNA作为模板,PCR 扩增HPV16 E6 全长基因,PCR 产物直接测序,进行突变分析.结果:本组维吾尔族妇女宫颈癌组织中HPV16的阳性率是73.6%(103/140),91例E6基因测序及分析结果表明,维吾尔族妇女宫颈癌组织中存在HPV16 E6变异株,其中49例(53.8%)分离株发生L83V突变,4例(4.39%)发生D25E突变,2例(2.20%)发生D64E/L83V突变,36例(39.6%)与野生型相同.结论:新疆维吾尔族妇女宫颈癌患者HPV16 E6基因发生变异,主要以L83V突变为主,HPV16变异株在维吾尔族妇女宫颈癌患者中的分布可能与维吾尔族妇女宫颈癌高发存在一定关系.

关 键 词:宫颈癌  人乳头状瘤病毒  E6基因  基因突变
文章编号:1009-0460(2008)03-0209-04
修稿时间:2007-08-13

Analysis of mutations in the E6 oncogene of human papillomavirus 16 in Xinjiang Uygur women with cervical carcinoma
Abstract:Objective:High-risk human papillomaviruses(HRHPVs),especially HPV16,is the major cause of cervical carcinoma(CC)in Xinjing Uygur women.In some region,specific E6 mutation is considered as dangerous factor causing CC.This study was designed to investigate distribution of HPV16 mutation and the relationship between the mutation and high incidence of CC in Uygur women.Methods:The tissue DNA was extracted from 140 CC paraffin embedded tissue of Uygur women.HPV16 E6 gene was amplified by polymerase chain reaction(PCR)from the CC tissue DNA.The PCR fragments were sequenced and analyzed.Results:The result of PCR showed that the positive rate of HPV16 was 73.5%(103/140);Ninty-one of 97 PCR fragments were sequenced and analyzed.Thirty-six of them maintained prototype(39.6%).Forty-nine have L83V mutation(53.6%)and 4 have D25E(4.25%),and 2 have L83V/D64E(2.27%).Conclusion:There are mutations within the HPV16 E6 gene in CC of Xinjiang Uygur women.The most common mutation was L83V.Our research suggested that the distribution of HPV16 E6 mutation might be associated with high incidence of CC in Uygur.
Keywords:Cervical carcinoma  Human papillomavirus  E6 gene  Gene mutation
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